LOCUS       Exported                3896 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector Sos_ZsG_pol I-mg, complete sequence.
ACCESSION   MG880223
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Welch SR, Chakrabarti AK, Wiggleton Guerrero L, Jenks HM, Lo MK, 
            Nichol ST, Spiropoulou CF, Albarino CG.
  TITLE     Development of a reverse genetics system for Sosuga virus allows 
            rapid screening of antiviral compounds
  JOURNAL   PLoS Negl Trop Dis 12 (3), e0006326 (2018)
  PUBMED    29522528
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Albarino C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2018) Viral Special Pathogens Branch, Centers for 
            Disease Control and Prevention, 1600 Clifton Rd. NE, Atlanta, GA 
            30329, USA
REFERENCE   3  (bases 1 to 3896)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Expression vector Sos_ZsG_pol I-mg"
                     /focus
                     /mol_type="other DNA"
                     /db_xref="taxon:2126431"
     source          36..960
                     /organism="Sosuga virus"
                     /mol_type="other DNA"
                     /db_xref="taxon:1452514"
     regulatory      2..35
                     /regulatory_class="terminator"
                     /note="pol I terminator"
     misc_feature    36..960
                     /label=SOSV/ZsG minigenome
                     /note="SOSV/ZsG minigenome"
     CDS             200..895
                     /codon_start=1
                     /product="ZsG"
                     /label=ZsG
                     /protein_id="AVT50845.1"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     regulatory      complement(962..1173)
                     /regulatory_class="promoter"
                     /note="pol I promoter"
     primer_bind     complement(1244..1260)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1268..1284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1292..1322)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1337..1358
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1646..2234)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2405..3265)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3266..3370)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3844..3860
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"