LOCUS       Exported                7730 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Lentiviral vector SCRPSY, complete sequence.
ACCESSION   KT368137
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Wilson SJ.
  TITLE     ISG expression-screening reveals multiple antiretroviral effectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7730)
  AUTHORS   Wilson SJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2015) MRC Centre for Virus Research, University of
            Glasgow, 464 Bearsden Road, Glasgow, Scotland G61 1QH, UK
REFERENCE   3  (bases 1 to 7730)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Lentiviral vector SCRPSY"
                     /mol_type="other DNA"
                     /db_xref="taxon:1868477"
     regulatory      4..590
                     /regulatory_class="promoter"
                     /note="CMV IE promoter"
     enhancer        64..367
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     repeat_region   584..764
                     /label=R and U5 of HIV-1 LTR
                     /note="R and U5 of HIV-1 LTR"
     LTR             584..764
                     /label=5 LTR (truncated)
                     /note="truncated 5 long terminal repeat (LTR) from HIV-1"
     misc_feature    811..936
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    992..1129
                     /label=Psi - packaging sequence
                     /note="Psi - packaging sequence"
     CDS             1349..2710
                     /codon_start=1
                     /product="PAC2ATagRFP"
                     /label=PAC2ATagRFP
                     /note="PAC (puromycin acetyl transferase) 2A 
                     (stop-start/skip from FMDV) TagRFP (red fluorescent 
                     protein)"
                     /protein_id="ANN89731.1"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAGSGEGRGSLLTCGDVEENPGPGS
                     MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKVVEGGPLPFAFDILA
                     TSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYN
                     VKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALKLVGGGHLICNFKTT
                     YRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYCDLPSKLGHK"
     CDS             1955..2008
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    2803..2920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             join(2984..3198,4178..4223)
                     /codon_start=1
                     /product="Tat"
                     /label=Tat
                     /protein_id="ANN89733.1"
                     /translation="MEPVDPRLEPWKHPGSQPKTACTNCYCKKCCFHCQVCFMTKALGI
                     SYGRKKRRQRRRAHQNSQTHQASLSKQPTSQSRGDPTGPKE"
     CDS             join(3123..3198,4178..4452)
                     /codon_start=1
                     /product="Rev"
                     /label=Rev
                     /protein_id="ANN89734.1"
                     /translation="MAGRSGDSDEELIRTVRLIKLLYQSNPPPNPEGTRQARRNRRRRW
                     RERQRQIHSISERILSTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQILV
                     ESPTILESGAKE"
     misc_feature    3568..3801
                     /label=RRE - Rev response element
                     /note="RRE - Rev response element"
     CDS             4324..4350
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     promoter        4475..4493
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_recomb     4523..4547
                     /label=attB1
                     /note="attB1"
     protein_bind    4523..4547
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     4615..4639
                     /label=attB2
                     /note="attB2"
     protein_bind    complement(4615..4639)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     repeat_region   4699..5334
                     /label=3 LTR
                     /note="3 LTR"
     LTR             4700..5334
                     /label=5 LTR
                     /note="5 long terminal repeat (LTR) from HIV-1"
     promoter        5443..5547
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      5478..5506
                     /regulatory_class="promoter"
                     /note="AmpR; ampicillin resistance gene; beta lactamase 
                     promoter"
     CDS             5548..6408
                     /codon_start=1
                     /product="beta-lactamase"
                     /function="confers ampicillin resistance"
                     /label=beta-lactamase
                     /protein_id="ANN89732.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7518..7714)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7532..7667
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      7590..7667
                     /label=SV40 origin
                     /note="SV40 origin"