LOCUS       Exported                5899 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Insertion vector scAAV-CMV-GFP, complete sequence.
ACCESSION   JX445134
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Rosas LE, Grieves JL, Zaraspe K, La Perle KM, Fu H, McCarty DM.
  TITLE     Patterns of scAAV Vector Insertion Associated With Oncogenic Events 
            in a Mouse Model for Genotoxicity
  JOURNAL   Mol. Ther. (2012) In press
  PUBMED    22990674
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Rosas LE, Grieves JL, Zaraspe K, La Perle KMD., Fu H, McCarty DM.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2012) Pediatrics/Center for Gene Therapy, Research
            Institute at Nationwide Childrens Hospital, 700 Childrens Dr, 
            Columbus, OH 43205, USA
REFERENCE   3  (bases 1 to 5899)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Insertion vector scAAV-CMV-GFP"
                     /mol_type="other DNA"
                     /db_xref="taxon:1234867"
     repeat_region   1..112
                     /label=AAV2 terminal repeat
                     /note="AAV2 terminal repeat"
     regulatory      complement(117..341)
                     /regulatory_class="polyA_signal_sequence"
                     /note="BGH"
     polyA_signal    202..313
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      complement(342..534)
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40"
     polyA_signal    405..539
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     gene            complement(549..1283)
                     /gene="GFP"
                     /label=GFP
     CDS             complement(552..1271)
                     /codon_start=1
                     /gene="GFP"
                     /product="GFP"
                     /label=GFP
                     /note="enhanced green fluorescent protein from Aequorea 
                     victoria"
                     /protein_id="AFS52245.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      1268..1277
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     intron          complement(1297..1492)
                     /note="SV40"
     intron          1348..1444
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     regulatory      complement(1561..2071)
                     /regulatory_class="promoter"
                     /note="CMV promoter"
     promoter        complement(1582..1785)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     repeat_region   2136..2272
                     /label=AAV2 terminal repeat
                     /note="AAV2 terminal repeat"
     misc_feature    2273..5889
                     /label=pEMBL plasmid
                     /note="pEMBL plasmid"
     rep_origin      2683..3196
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3942..4046
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4047..4907
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5078..5666
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"