LOCUS       Exported                4739 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Transposition vector RescueMu, complete sequence.
ACCESSION   AY301066
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Raizada MN, Nan GL, Walbot V.
  TITLE     Somatic and germinal mobility of the RescueMu transposon in 
            transgenic maize
  JOURNAL   Plant Cell 13 (7), 1587-1608 (2001)
  PUBMED    11449053
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Walbot V.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2003) Biological Sciences, Stanford University, 
            385 Serra Mall, Stanford, CA 94305-5020, USA
REFERENCE   3  (bases 1 to 4739)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Transposition vector RescueMu"
                     /mol_type="other DNA"
                     /db_xref="taxon:234691"
     repeat_region   1..210
                     /label=TIR
                     /note="TIR"
     repeat_region   454..560
     primer_bind     761..777
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    784..1216
                     /label=derived from rhizobium
                     /note="derived from rhizobium"
     primer_bind     complement(1213..1229)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1217..4111
                     /label=derived from pBlueScript vector
                     /note="derived from pBlueScript vector"
     promoter        complement(1262..1280)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1287..1303)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(1444..1899)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1926..2030
                     /gene="bla"
                     /label=AmpR promoter
     gene            2031..2893
                     /gene="bla"
                     /label=bla
     CDS             2031..2891
                     /codon_start=1
                     /gene="bla"
                     /product="beta lactamase"
                     /label=bla
                     /protein_id="AAP69524.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3062..3650
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3938..3959
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3974..4004
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4012..4028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4036..4052
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4073..4091
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     mobile_element  4105..743
                     /mobile_element_type="transposon:Mu1"
                     /note="derived from Zea mays"
     repeat_region   4220..4323
     repeat_region   4532..4739
                     /label=TIR
                     /note="TIR"