LOCUS       Exported               11572 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector RCAS-L14 DNA, complete sequence.
ACCESSION   AB105370
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11572)
  AUTHORS   Komaguchi C, Kawakami Y, Nohno T.
  TITLE     Nucleotide sequence of retroviral cloning vector, RCAS-L14
  JOURNAL   Published Only in Database (2003)
REFERENCE   2  (bases 1 to 11572)
  AUTHORS   Nohno T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2003) Tsutomu Nohno, Kawasaki Medical School, 
            Department of Molecular Biology; 577 Matsushima, Kurashiki, Okayama 
            701-0192, Japan (E-mail:nohno@bcc.kawasaki-m.ac.jp, 
            Tel:81-86-462-1111(ex.83501), Fax:81-86-462-1199)
REFERENCE   3  (bases 1 to 11572)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11572
                     /organism="Cloning vector RCAS-L14"
                     /mol_type="other DNA"
                     /db_xref="taxon:224009"
     misc_feature    1..4
                     /label=pBR322-derived sequence
                     /note="pBR322-derived sequence"
     repeat_region   139..438
     promoter        139..400
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             739..2850
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein"
                     /label=gag
                     /protein_id="BAC65349.1"
                     /translation="MEAVIKVISSACKTYCGKISPSKKEIGAMLSLLQKEGLLMSPSDL
                     YSPGSWDPITAALSQRAMVLGKSGELKTWGLVLGALKAAREEQVTSEQAKFWLGLGGGR
                     VSPPGPECIEKPATERRIDKGEEVGETTVQRDAKMAPEEAATPKTVGTSCYHCGTAVGC
                     NCATATASAPPPPYVGSGLYPSLAGVGEQQGQGDNTSRGAEQPREEPGHAGQAPGPALT
                     DWARVREELASTGPPVVAMPVVIKTEGPAWTPLEPKLITRLADTVRTKGLRSPITMAEV
                     EALMSSPLLPHDVTNLMRVILGPAPYALWMDAWGVQLQTVIAAATRDPRHPANGQGRGE
                     RTNLDRLKGLADGMVGNPQGQAALLRPGELVAITASALQAFREVARLAEPAGPWADITQ
                     GPSESFVDFANRLIKAVEGSDLPPSARAPVIIDCFRQKSQPDIQQLIRAAPSTLTTPGE
                     IIKYVLDRQKIAPLTDQGIAAAMSSAIQPLVMAVVNRERDGQTGSGGRARGLCYTCGSP
                     GHYQAQCPKKRKSGNSRERCQLCDGMGHNAKQCRKRDGNQGQRPGRGLSSGPWPGPEQP
                     AVSLAMTMEHKDRPLVRVILTNTGSHPVKQRSVYITALLDSGADITIISEEDWPTDWPV
                     VDTANPQIHGIGGGIPMRKSRDMIEVGVINRDGSLERPLLLFPAVAMVRGSILGRDCLQ
                     GLGLRLTNL"
     gene            739..2850
                     /gene="gag"
                     /label=gag
     CDS             2868..5555
                     /codon_start=1
                     /gene="pol"
                     /product="reverse transcriptase"
                     /label=pol
                     /protein_id="BAC65350.1"
                     /translation="TVALHLAIPLKWKSDRTPVWIDQWPLPEGKLVALTQLVEKELQLG
                     HIEPSLSCWNTPVFVIRKASGSYRLLHDLRAVNAKLVPFGAVQQGAPVLSALPRGWPLM
                     VLDLKDCFFSIPLAEQDREAFAFTLPSVNNQAPARRFQWKVLPQGMTCSPTICQLVVGQ
                     VLEPLRLKHPALRMLHYMDDLLLAASSHDGLEAAGKEVIGTLERAGFTISPDKIQREPG
                     VQYLGYKLGSTYVAPVGLVAEPRIATLWDVQKLVGSLQWLRPALGIPPRLMGPFYEQLR
                     GSDPNEAREWNLDMKMAWREIVQLSTTAALERWDPAQPLEGAVARCEQGAIGVLGQGLS
                     THPRPCLWLFSTQPTKAFTAWLEVLTLLITKLRASAVRTFGKEVDILLLPACFREDLPL
                     PEGILLALRGFAGKIRSSDTPSIFDIARPLHVSLKVRVTDHPVPGPTVFTDASSSTHKG
                     VVVWREGPRWEIKEIVDLGASVQQLEARAVAMALLLWPTTPTNVVTDSAFVAKMLLKMG
                     QEGVPSTAAAFILEDALSQRSAMAAVLHVRSHSEVPGFFTEGNDVADSQATFQAYPLRE
                     AKDLHTALHIGPRALSKACNISMQQAREVVQTCPHCNSAPALEAGVNPRGLGPLQIWQT
                     DFTLEPRMAPRSWLAVTVDTASSAIVVTQHGRVTSVAAQHHWATAIAVLGRPKAIKTDN
                     GSCFTSKSTREWLARWGIAHTTGIPGNSQGQAMVERANRLLKDKIRVLAEGDGFMKRIP
                     ASKQGELLAKAMYALNHFERGENTKTPVQKHWRPTVLTEGPPVKIRIETGEWEKGWNVL
                     VWGRGYAAVKNRDTDKVIWVPSRKVKPDVTQKDEVTKKDEASPLFAGISDWIPWEDEQE
                     GLQGETASNKQERPGEDTLAANES"
     gene            2868..5555
                     /gene="pol"
                     /label=pol
     CDS             5443..7248
                     /codon_start=1
                     /gene="env"
                     /product="envelope protein"
                     /label=env
                     /protein_id="BAC65351.1"
                     /translation="AFLTGYPGKTSKKDSKEKPLATSKKDPEKTPLLPTRVNYILIIGV
                     LVLCEVTGVRADVHLLEQPGNLWITWANRTGQTDFCLSTQSATSPFQTCLIGIPSPISE
                     GDFKGYVSDTNCTTLGTDRLVSSADFTGGPDNSTTLTYRKVSCLLLKLNVSMWDEPPEL
                     QLLGSQSLPNITNIAQISGITGGCVGFRPQGVPWYLGWSRQEATRFLLRHPSFSKSTEP
                     FTVVTADRHNLFMGSEYCGAYGYRFWNMYNCSQVGRQYRCGNARSPRPGLPEIQCTRRG
                     GKWVNQSQEINESEPFSFTVNCTASSLGNASGCCGKAGTILPGKWVDSTQGSFTKPKAL
                     PPAIFLICGDRAWQGIPSRPVGGPCYLGKLTMLAPKHTDILKVLVNSSRTGIRRKRSTS
                     HLDDTCSDEVQLWGPTARIFASILAPGVAAAQALREIERLACWSVKQANLTTSLLGDLL
                     DDVTSIRHAVLQNRAAIDFLLLAHGHGCEDVAGMCCFNLSDQSESIQKKFQLMKEHVNK
                     IGVDSDLIGSWLRGLFGGIGEWAVHLLKGLLLGLVVILLLVVCLPCLLQMLCGNRRKMI
                     NNSISYHTEYKKLQKACGQPESRIV"
     gene            5443..7248
                     /gene="env"
                     /label=env
     misc_feature    7387..7421
                     /label=polylinker sequence
                     /note="polylinker sequence"
     promoter        7558..7819
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     repeat_region   7558..7807
     misc_feature    8043..11572
                     /label=pBR322-derived sequence
                     /note="pBR322-derived sequence"
     promoter        complement(8384..8412)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        8525..8629
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8630..9490
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="BAC65352.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            8630..9490
                     /gene="bla"
                     /label=bla
     rep_origin      9661..10249
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    10435..10575
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(10677..10868)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"