LOCUS       Exported                6733 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  E. Coli/Gram-positive Bacterial Shuttle Vector.
ACCESSION   .
VERSION     .
KEYWORDS    pDL278
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   LeBlanc DJ, Lee LN, Abu-Al-Jaibat A
  TITLE     Molecular, genetic, and functional analysis of the basic replicon of
            pVA380-1, a plasmid of oral streptococcal origin.
  JOURNAL   Plasmid. 1992 Sep;28(2):130-45.
  PUBMED    1409970
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid."; 
            date: "1992-09"; volume: "28(2)"; pages: "130-45"
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(66..83)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(237..825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(317..336)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      5155..5182
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      5274..5360
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5653..5680
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      5772..5858
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(6024..6422)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGECRELFTTPRIGANQFLRRTVNAQTNPWQ
                     NISIASAISSSRTRRISAVLADERRFSA"
     primer_bind     6317..6339
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6331..6348
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6332..6348
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6349..6405
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(6418..6434)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6418..6434)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6431..6453)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6442..6458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6466..6496)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6511..6532
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."