LOCUS       Exported                6876 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pZX9, complete sequence.
ACCESSION   KC747117
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6876)
  AUTHORS   Xie Z, Qi F, Merritt J.
  TITLE     Development of a tunable wide-range gene induction system useful for
            the study of streptococcal toxin-antitoxin systems
  JOURNAL   Appl. Environ. Microbiol. 79 (20), 6375-6384 (2013)
  PUBMED    23934493
REFERENCE   2  (bases 1 to 6876)
  AUTHORS   Xie Z, Merritt J.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2013) Microbiology and Immunology, University of 
            Oklahoma Health Sciences Center, 975 NE 10th St BRC364, Oklahoma 
            City, OK 73104, USA
REFERENCE   3  (bases 1 to 6876)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6876
                     /organism="Expression vector pZX9"
                     /mol_type="other DNA"
                     /note="derived from the Escherichia coli-Streptococcus 
                     shuttle vector pDL278"
                     /db_xref="taxon:1302305"
     CDS             276..1043
                     /codon_start=1
                     /product="spectinomycin adenyltransferase"
                     /label=spectinomycin adenyltransferase
                     /note="Aad9"
                     /protein_id="AGH58085.1"
                     /translation="MRRIYLNTYEQINKVKKILRKHLKNNLIGTYMFGSGVESGLKPNS
                     DLDFLVVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIELTIIIQQEMVPWNHPPKQ
                     EFIYGEWLQELYEQGYIPQKELNSDLTIMLYQAKRKNKRIYGNYDLEELLPDIPFSDVR
                     RAIMDSSEELIDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERI
                     LLAVRSYLGENIEWTNENVNLTINYLNNRLKKL"
     terminator      3749..3776
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(3832..5484)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=firefly luciferase
                     /note="Luc"
                     /protein_id="AGH58086.1"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     CDS             5710..6876
                     /codon_start=1
                     /product="xylose repressor"
                     /label=xylose repressor
                     /note="derived from Bacillus megaterium sequence; XylR"
                     /protein_id="AGH58087.1"
                     /translation="MVIIQIADQALVKKMNQKLILDEILKNSPVSRATLSEITGLNKST
                     VSSQVNTLLEKDFIFEIGAGQSRGGRRPVMLVFNKNAGYSIGIDIGVDYLNGILTDLEG
                     NIILEKTSDLSSSSASEVKEILFALIHGFVTHMPESPYGLVGIGICVPGLVDRHQQIIF
                     MPNLNWNIKDLQFLIESEFNVPVFVENEANAGAYGEKVFGMTKNYENIVYISINIGIGT
                     GLVINNELYKGVQGFSGEMGHMTIDFNGPKCSCGNRGCWELYASEKALLASLSKEEKNI
                     SRKEIVERANKNDVEMLNALQNFGFYIGIGLTNILNTFDIEAVILRNHIIESHPIVLNT
                     IKNEVSSRVHSHLDNKCELLPSSLGKNAPALGAVSIVIDSFLSVTPIS"