LOCUS       Exported                8349 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Promoter probe vector pZWHJ002, complete sequence.
ACCESSION   KC465961
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Chao H, Zhou NY.
  TITLE     GenR, an IclR-Type Regulator, Activates and Represses the 
            Transcription of gen Genes Involved in 3-Hydroxybenzoate and 
            Gentisate Catabolism in Corynebacterium glutamicum
  JOURNAL   J. Bacteriol. 195 (7), 1598-1609 (2013)
  PUBMED    23354754
REFERENCE   2  (bases 1 to 8349)
  AUTHORS   Chao H-J., Zhou N-Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2013) Key Laboratory of Agricultural and 
            Environmental Microbiology, Wuhan Institute of Virology, Chinese 
            Academy of Sciences, No. 44 Xiao Hong Shan, Wu Han, Hubei 430071, 
            P.R. China
REFERENCE   3  (bases 1 to 8349)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8349
                     /organism="Promoter probe vector pZWHJ002"
                     /mol_type="other DNA"
                     /note="Corynebacterium glutamicum/Escherichia coli shuttle 
                     vector; promoter-probe vector using lacZ as a reporter 
                     gene; created by replacing lacI and Ptac with a 3.1 kb lacZ
                     fragment of Escherichia coli Rosetta (DE3) pLysS at the 
                     EheI and PstI (Fermentas) sites of plasmid pXMJ19"
                     /db_xref="taxon:1308864"
     misc_feature    1..57
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     misc_feature    1..45
                     /label=multiple cloning site (MCS)
                     /note="multiple cloning site (MCS)"
     regulatory      63..488
                     /regulatory_class="terminator"
                     /note="rrnB"
     terminator      265..351
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      443..470
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        489..580
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      963..1535
                     /label=ori pUC
                     /note="ori pUC"
     rep_origin      complement(967..1555)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      1720..4274
                     /label=ori pBL1
                     /note="ori pBL1"
     CDS             complement(4406..5065)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyl transferase"
                     /EC_number="2.3.1.28"
                     /label=cat
                     /protein_id="AGI05084.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(4406..5065)
                     /gene="cat"
                     /label=cat
     regulatory      complement(5131..5225)
                     /regulatory_class="promoter"
                     /note="psiGA1"
     CDS             complement(5270..8344)
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-D-galactosidase"
                     /EC_number="3.2.1.23"
                     /label=lacZ
                     /protein_id="AGI05085.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(5270..8344)
                     /gene="lacZ"
                     /label=lacZ
     primer_bind     8308..8324
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"