LOCUS       Exported                4459 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pZT90 DNA, complete sequence.
ACCESSION   AB473551
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Satoh K, Tu Z, Ohba H, Narumi I.
  TITLE     Development of versatile shuttle vectors for Deinococcus grandis
  JOURNAL   Plasmid 62 (1), 1-9 (2009)
  PUBMED    19504653
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Narumi I.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2008) Contact:Issay Narumi Japan Atomic Energy 
            Agency, Quantum Beam Science Directorate; 1233 Watanuki, Takasaki, 
            Gunma 370-1292, Japan
REFERENCE   3  (bases 1 to 4459)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Expression vector pZT90"
                     /lab_host="Deinococcus grandis"
                     /mol_type="other DNA"
                     /note="assembled from Deinococcus radiopugnans plasmid 
                     pUE30 and chloramphenicol acetyltansferase gene supply 
                     plasmid pKatCAT5 with Deinococcus radiodurans groE 
                     promoter"
                     /db_xref="taxon:582884"
     CDS             205..1710
                     /codon_start=1
                     /gene="orf2"
                     /label=orf2
                     /note="Orf2; similar to Deinococcus radiodurans plasmid 
                     pUE10 RepU"
                     /protein_id="BAH03366.1"
                     /translation="MAQKISREGGAGPAVHPNWQHPAGRRGAKVILEASQKCLQTPLIE
                     PSHSEGPLPMNNQKQDSYTDRVRQIAPVVPLLDLIVKPLIPERAAPNPYLEMGLAALRG
                     EPVDTRRAQVQPSKQFQKASRPAPAAPPLVLTSDQGEDQEAAMLLPEIVEETRRRLEKQ
                     ARENRETRRAEQRARFHQLVTAPTDEAAPDFVPPVSLQEGRQPPSGSVRVTLPRPEVPG
                     PAWGELSADAVLDSVPWLSKGARLVFRIMHMLAVAKAQDCRYPVIPNSAAFHTPQLLLA
                     FVARYTTRHFRRLTNELEHAGVIDGGAHAAKVKTGIGTTRHLWDGSIWAVKLRPSTCDA
                     YLSPEDWQHEWRDFQADLESGRTAKKLMSYLNTCEGLARQEHVLKTWAVNPNAKMTSLC
                     LERTFQAEQKMTLQDTVYALPLIGELSELKQAGAIGNAASIISHALGDSHSRRFWCGLL
                     WAATRNGSLEAFSAQLLRLLADVQESSELRNPGALFAARLRSA"
     gene            205..1710
                     /gene="orf2"
                     /label=orf2
     misc_feature    1778..2060
                     /note="region of low %G+C (36%); possibly involved in 
                     replication initiation"
     primer_bind     complement(2123..2139)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2356..2944
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3232..3253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3268..3298
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3306..3322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3330..3346
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3495..4154)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /EC_number="2.3.1.28"
                     /label=cat
                     /note="confer resistance to chloramphenicol"
                     /protein_id="BAH03367.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(3495..4154)
                     /gene="cat"
                     /label=cat
     regulatory      complement(4212..4240)
                     /regulatory_class="promoter"
                     /note="derived from Deinococcus radiodurans katA promoter"
     misc_feature    4288..4293
                     /label=EcoT22I site
                     /note="EcoT22I site"
     regulatory      complement(4379..4408)
                     /regulatory_class="promoter"
                     /note="derived from Deinococcus radiodurans groE promoter"
     primer_bind     complement(4427..4443)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"