LOCUS       Exported                6405 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pZR80, complete sequence.
ACCESSION   AY003885
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Kok RG, Young DM, Ornston LN.
  TITLE     Phenotypic expression of PCR-generated random mutations in a 
            Pseudomonas putida gene after its introduction into an Acinetobacter
            chromosome by natural transformation
  JOURNAL   Appl. Environ. Microbiol. 65 (4), 1675-1680 (1999)
  PUBMED    10103267
REFERENCE   2  (bases 1 to 6405)
  AUTHORS   Young DM, Kok RG, Ornston L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2000) Molecular, Cellular, and Developmental 
            Biology, Yale University, P.O. Box 208103 Room KBT 840, New Haven, 
            CT 06520, USA
REFERENCE   3  (bases 1 to 6405)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Cloning vector pZR80"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:130970"
     misc_feature    1..6405
                     /note="designed to allow cloning of a foreign gene into a 
                     polylinker that is flanked by DNA possessing homology the 
                     Acinetobacter ADP1; a gene cloned into the vector can be 
                     inserted into an Acinetobacter recipient strain by natural 
                     transformation and recombination"
     CDS             571..1602
                     /codon_start=1
                     /gene="lipB"
                     /product="lipase foldase"
                     /label=lipB
                     /note="LipB; chaperone"
                     /protein_id="AAF86566.1"
                     /translation="MNGSKKIYLGIGLVALLMIFIYWLMPKDTANASSQIESTNASAII
                     ATSPGQQNQLSENTTPFGSVSQHDTQVNCQLQLNAANHLIVNEQTRNCFEYFLTQYGEK
                     SLTQIDQDIKNYFTQSLPQPARDQAQDLWQRYLKYREELGNLKEPAIAKTDIAYYRAVF
                     TSRQMLRQRFFSATEIAGLFGSEDIYNQYTLERMAILNNSKLSEIEKAKQLKALFDQLP
                     QDWKANLEQLSKLDDLKQLTTSIKKNGGSAQELHDMRTNLVGHDATARLEQLDVERSNW
                     KSNVTQYLDERQTILNSNMSDTAKQNAISALRSKNFTAPQVQIRVQAFESAKDQGQSLP
                     FSE"
     gene            571..1602
                     /gene="lipB"
                     /label=lipB
     regulatory      1749..1777
                     /regulatory_class="promoter"
                     /note="Ptet"
     promoter        1749..1777
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     misc_feature    1929..1985
                     /label=polylinker
                     /note="polylinker"
     regulatory      2007..2012
                     /regulatory_class="promoter"
                     /note="PaphA3"
     CDS             2069..2863
                     /codon_start=1
                     /gene="aphA"
                     /allele="aphA-3"
                     /product="aminoglycoside phosphotransferase A3"
                     /function="kanamycin resistance"
                     /label=aphA
                     /protein_id="AAF86567.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            2069..2863
                     /gene="aphA"
                     /allele="aphA-3"
                     /label=aphA
     misc_feature    2929..3096
                     /label=truncated lipA
                     /note="truncated lipA"
     rep_origin      complement(4568..5156)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5327..6187)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="ampicillin resistance"
                     /label=bla
                     /protein_id="AAF86568.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(5327..6187)
                     /gene="bla"
                     /label=bla
     promoter        complement(6188..6292)
                     /gene="bla"
                     /label=AmpR promoter