LOCUS       Exported                8411 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Integration vector pZR606, complete sequence.
ACCESSION   KJ500179
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8411)
  AUTHORS   Chen K, Xu X, Gu L, Hildreth M, Zhou R.
  TITLE     Simultaneous gene inactivation and promoter reporting in 
            cyanobacteria
  JOURNAL   Appl. Microbiol. Biotechnol. 99 (4), 1779-1793 (2015)
  PUBMED    25434810
REFERENCE   2  (bases 1 to 8411)
  AUTHORS   Chen K, Xu X, Gu L, Zhou R.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2014) Biology and Microbiology, South Dakota State
            University, 1110 Rotunda Ln. N, Brookings, SD 57007, USA
REFERENCE   3  (bases 1 to 8411)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8411
                     /organism="Integration vector pZR606"
                     /mol_type="other DNA"
                     /db_xref="taxon:1496939"
     CDS             complement(473..1894)
                     /codon_start=1
                     /gene="sacB"
                     /product="SacB"
                     /label=sacB
                     /note="derived from Bacillus subtilis"
                     /protein_id="AHZ10951.1"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLEVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     gene            complement(473..1894)
                     /gene="sacB"
                     /label=sacB
     promoter        complement(1895..2340)
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             2749..3543
                     /codon_start=1
                     /gene="nptII"
                     /product="NptII"
                     /label=nptII
                     /note="kanamycin and neomycin resistance determinant"
                     /protein_id="AHZ10953.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            2749..3543
                     /gene="nptII"
                     /label=nptII
     misc_feature    3849..3883
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             3910..4629
                     /codon_start=1
                     /gene="gfp-uv"
                     /product="GFPuv"
                     /label=gfp-uv
                     /protein_id="AHZ10954.1"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     gene            3910..4629
                     /gene="gfp-uv"
                     /label=gfp-uv
     CDS             5449..6240
                     /codon_start=1
                     /gene="aadA"
                     /product="AadA"
                     /label=aadA
                     /note="streptomycin and specinomycin resistance 
                     determinant"
                     /protein_id="AHZ10952.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            5449..6240
                     /gene="aadA"
                     /label=aadA
     primer_bind     complement(6583..6599)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      7356..7944
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    8130..8270
                     /label=bom
                     /note="basis of mobility region from pBR322"