LOCUS       Exported                8489 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector PZPnat1, complete sequence.
ACCESSION   AY631958
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Gardiner DM, Howlett BJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2004) School of Botany, The University of 
            Melbourne, Melbourne, VIC 3010, Australia
REFERENCE   2  (bases 1 to 8489)
  AUTHORS   Gardiner DM, Howlett BJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2014) School of Botany, The University of 
            Melbourne, Melbourne, VIC 3010, Australia
REFERENCE   3  (bases 1 to 8489)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Mar 13, 2014 this sequence version replaced AY631958.1.
FEATURES             Location/Qualifiers
     source          1..8489
                     /organism="Cloning vector PZPnat1"
                     /mol_type="other DNA"
                     /db_xref="taxon:282076"
     repeat_region   94..119
                     /label=right border repeat
                     /note="right border repeat"
     misc_feature    114..138
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     334..350
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     380..396
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      440..801
                     /regulatory_class="promoter"
                     /note="TrpC"
     CDS             818..1387
                     /codon_start=1
                     /product="nourseothricin acetyl transferase"
                     /label=nourseothricin acetyl transferase
                     /note="NatR"
                     /protein_id="AAT51694.2"
                     /translation="MTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGFT
                     LREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNRR
                     LTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFTL
                     CGLDTALYDGTASDGEQALYMSMPCP"
     regulatory      1398..2115
                     /regulatory_class="terminator"
                     /note="TrpC"
     primer_bind     complement(2137..2153)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2161..2177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2185..2215)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2230..2251
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   2427..2452
                     /label=left border repeat
                     /note="left border repeat"
     misc_feature    2428..2452
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2745..3560
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=aminoglycoside phosphotransferase
                     /note="KanR"
                     /protein_id="AAT51695.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3655..4243
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      3859..4139
                     /label=pBR322
                     /note="pBR322"
     misc_feature    4429..4569
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      4913..5107
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     rep_origin      4949..5949
                     /label=pVS1
                     /note="pVS1"
     CDS             complement(5173..6240)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     misc_feature    6542..7542
                     /label=STA region from pVS1
                     /note="STA region from pVS1"
     CDS             complement(6674..7303)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"