LOCUS       Exported                5933 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pZJY156, complete sequence.
ACCESSION   EU328350
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Zhao JY, Zhong L, Shen MJ, Xia ZJ, Cheng QX, Sun X, Zhao GP, Li YZ, 
            Qin ZJ.
  TITLE     Discovery of the autonomously replicating plasmid pMF1 from 
            Myxococcus fulvus and development of a gene cloning system in 
            Myxococcus xanthus
  JOURNAL   Appl. Environ. Microbiol. 74 (7), 1980-1987 (2008)
  PUBMED    18245244
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Zhao J, Zhong L, Qin Z, Li Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2007) State Key Laboratory of Microbial 
            Technology, School of Life Science, Shandong University, 27 South 
            Road of Shanda, Jinan, Shandong 250100, Peoples Republic of China
REFERENCE   3  (bases 1 to 5933)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Shuttle vector pZJY156"
                     /lab_host="Myxococcus xanthus"
                     /mol_type="other DNA"
                     /label=Escherichia coli-Myxococcus shuttle vector
                     /note="Escherichia coli-Myxococcus shuttle vector"
                     /db_xref="taxon:498499"
     CDS             296..1090
                     /codon_start=1
                     /gene="aphII"
                     /product="aminoglycoside-3-O-phosphotransferase"
                     /label=aphII
                     /protein_id="ABY83641.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            296..1090
                     /gene="aphII"
                     /label=aphII
     CDS             1429..3333
                     /codon_start=1
                     /gene="rep-pMF1"
                     /product="putative Rep-pMF1 protein"
                     /label=rep-pMF1
                     /protein_id="ABY83642.1"
                     /translation="MKRRALTSQPTPARALVHGAGSGERPTLREPSRAKSQPRNRRSGL
                     DVEPSAASASSLPADQVAQLRASTARLRREQSQRQRRVNAGKARRNEERKWAALELATG
                     TALPLVDTVGFRPAEVSKPETYVTLEDTHGLEGLARGERAEQELEAFRASHPSRQVAPP
                     AALAIAAILYSTRKAEGGADARDCASLGWQVPVRGWCEMTGYRERAVQAAFAWLADEGL
                     VRRFGDYVTVKHLTRLRPAGERLDLWLDAKGKARRWVQVYSVTYLTNAGAAWLERAGFD
                     LAETRGHGVAWRRRGFLARVGRALAGLWRTVRRRLGGGGSVAVGAGATCTPYPAKQVEV
                     CSRRGDDSQPTASLEELIGAESPPHGGDVTGSASAKPDPRAANAEHARVEGAPAQGGHA
                     SMGSASAKLEGSKPPPSALGPEWEKKLGLTAEGIAAGLAWEGGGLLAYAEVVDRCWTRT
                     TTTTRAAPSDVPSGSRNMFGRRRVWRCRGCGAESRYKGATVRHAQGCTAVGAWHLARGP
                     AVETWSPAMTAELAWPEDWEAHPELRPRLRRLFVPVAAELTRRIQARAAEARRAAEAEE
                     AERRHLEVWMSKHPECGGAFRAPVYDSSVCVADGPPNAWGRAPLCYCDACSQKRRDIE"
     gene            1429..3333
                     /gene="rep-pMF1"
                     /label=rep-pMF1
     promoter        complement(3571..3589)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3847..4435)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4606..5466)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="ABY83640.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(4606..5466)
                     /gene="bla"
                     /label=bla
     promoter        complement(5467..5571)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5917..2
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"