LOCUS       Exported                5231 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pZJ25, complete sequence.
ACCESSION   KP057690
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Zhou J, Olson DG, Lanahan AA, Tian L, Murphy SJ, Lo J, Lynd LR.
  TITLE     Physiological roles of pyruvate ferredoxin oxidoreductase and 
            pyruvate formate-lyase in Thermoanaerobacterium saccharolyticum 
            JW/SL-YS485
  JOURNAL   Biotechnol Biofuels 8, 138 (2015)
  PUBMED    26379770
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Zhou J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2014) Thayer School of Engineering, Dartmouth 
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 5231)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Cloning vector pZJ25"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:1619669"
     CDS             7..744
                     /codon_start=1
                     /gene="ermR"
                     /product="ErmR"
                     /label=ermR
                     /protein_id="AJP77050.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
                     KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYH
                     LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH
                     PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN
                     LSTVTYEQVLSIFNSYLLFNGRK"
     gene            7..744
                     /gene="ermR"
                     /label=ermR
     misc_feature    802..1704
                     /label=upstream of Tsac_0628
                     /note="upstream of Tsac_0628"
     rep_origin      1947..2535
                     /label=pMB1 ori from pUC19
                     /note="pMB1 ori from pUC19"
     CDS             complement(2706..3566)
                     /codon_start=1
                     /product="AmpR"
                     /label=AmpR
                     /note="Ap(r) determinant; derived from pUC19"
                     /protein_id="AJP77049.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3567..3671)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    3765..4680
                     /label=downstream of Tsac_0629
                     /note="downstream of Tsac_0629"
     regulatory      4688..5231
                     /regulatory_class="promoter"
                     /note="kanamycin promoter from pMU433"