LOCUS       Exported                5832 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pZH3, complete sequence.
ACCESSION   AF168612
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Hu JC.
  TITLE     Repressor fusions as a tool to study protein-protein interactions
  JOURNAL   Structure 3 (5), 431-433 (1995)
  PUBMED    7663940
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Zhu H, Celinski SA, Scholtz JM, Hu JC.
  TITLE     The contribution of buried polar groups to the conformational 
            stability of the GCN4 coiled coil
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5832)
  AUTHORS   Zhu H, Celinski SA, Scholtz JM, Hu JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUL-1999) Biochemistry & Biophysics, Texas A&M 
            University, MS2128, College Station, TX 77842-2128, USA
REFERENCE   4  (bases 1 to 5832)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Expression vector pZH3"
                     /lab_host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /note="designed for expression of leucine zipper peptides; 
                     expression requires strains expressing the phage T7 RNA 
                     polymerase"
                     /db_xref="taxon:101771"
     promoter        12..40
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      408..455
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    517..2528
                     /label=stuffer fragment from Escherichia coli lacZ
                     /note="stuffer fragment from Escherichia coli lacZ"
     CDS             complement(2497..2535)
                     /codon_start=1
                     /product="expressed peptide start"
                     /label=expressed peptide start
                     /protein_id="AAD50824.1"
                     /translation="MSTDRSRRFTTS"
     RBS             2543..2565
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    2545..516
                     /label=derived from plasmid pET3a
                     /note="derived from plasmid pET3a"
     promoter        complement(2597..2615)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2599..2616
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     CDS             3386..3577
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    3679..3821
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4007..4595)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4766..5626)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5627..5731)
                     /gene="bla"
                     /label=AmpR promoter