LOCUS       Exported               12329 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pZH2Bik DNA, complete sequence.
ACCESSION   AB551245
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12329)
  AUTHORS   Kuroda M, Kimizu M, Mikami C.
  TITLE     A simple set of plasmids for the production of transgenic plants
  JOURNAL   Biosci. Biotechnol. Biochem. 74 (11), 2348-2351 (2010)
  PUBMED    21071849
REFERENCE   2  (bases 1 to 12329)
  AUTHORS   Kuroda M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2010) Contact:Masaharu Kuroda National 
            Agricultural Research Center, Rice Physiology Research Team; Inada 
            1-2-1, Joetsu, Niigata 943-0193, Japan
REFERENCE   3  (bases 1 to 12329)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Mar 4, 2011 this sequence version replaced AB551245.1.
FEATURES             Location/Qualifiers
     source          1..12329
                     /organism="Binary vector pZH2Bik"
                     /mol_type="other DNA"
                     /db_xref="taxon:751899"
     CDS             1187..1816
                     /codon_start=1
                     /gene="staA"
                     /product="pVS1 partitioning protein"
                     /label=staA
                     /inference="similar to DNA sequence:same 
                     species:AF118810.1"
                     /protein_id="BAJ22057.1"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     gene            1187..1816
                     /gene="staA"
                     /label=staA
     CDS             2245..3318
                     /codon_start=1
                     /gene="repA"
                     /product="pVS1 replication protein"
                     /label=repA
                     /inference="similar to DNA sequence:same 
                     species:AF118810.1"
                     /protein_id="BAJ83552.1"
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     gene            2245..3318
                     /gene="repA"
                     /label=repA
     rep_origin      3384..3578
                     /label=derived from pVS1
                     /note="derived from pVS1"
                     /inference="similar to DNA sequence:same 
                     species:AF118810.1"
     misc_feature    3922..4062
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4248..4836)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      4248..4835
                     /label=ColE1-type, derived from pBR322
                     /note="ColE1-type, derived from pBR322"
                     /inference="similar to DNA sequence:same species:V00268.1"
     CDS             complement(5080..5871)
                     /codon_start=1
                     /gene="aadA"
                     /product="spectiomycin resistance protein"
                     /label=aadA
                     /protein_id="BAJ22058.1"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(5080..5871)
                     /gene="aadA"
                     /label=aadA
     misc_feature    6397..6421
                     /label=T-DNA left border
                     /note="T-DNA left border"
     misc_feature    6399..6423
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     6638..6654
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      6671..7516
                     /regulatory_class="promoter"
                     /gene="CaMV35S"
     gene            6671..7516
                     /gene="CaMV35S"
                     /label=CaMV35S
     promoter        7162..7507
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             7523..8548
                     /codon_start=1
                     /gene="HPH"
                     /product="modified hygromycin phosphotransferase"
                     /label=HPH
                     /protein_id="BAJ22059.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEVMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     gene            7523..8548
                     /gene="HPH"
                     /label=HPH
     gene            8570..11890
                     /gene="Nos"
                     /label=Nos
     regulatory      8570..8828
                     /regulatory_class="terminator"
                     /gene="Nos"
     regulatory      8843..10590
                     /regulatory_class="promoter"
                     /gene="Nos"
                     /note="derived from rice, ubi1 gene promoter with first 
                     intron"
     misc_feature    10615..11598
                     /gene="Nos"
                     /note="derived from rice, third intron of aspartic protease
                     coding gene"
     regulatory      11626..11890
                     /regulatory_class="terminator"
                     /gene="Nos"
     terminator      11636..11888
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(11920..11936)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11944..11960
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11968..11998)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12013..12034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    12190..12214
                     /label=T-DNA right border
                     /note="T-DNA right border"
     misc_feature    12192..12216
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"