LOCUS       Exported                5868 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pZeoSv2-Furin, complete sequence.
ACCESSION   KF886270
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Liu J, Jonebring A, Hagstrom J, Nystrom AC, Lovgren A.
  TITLE     Improved Expression of Recombinant Human Factor IX by Co-expression 
            of GGCX, VKOR and Furin
  JOURNAL   Protein J. 33 (2), 174-183 (2014)
  PUBMED    24567122
REFERENCE   2  (bases 1 to 5868)
  AUTHORS   Liu J, Jonebring A, Hagstrom J, Nystrom A-C., Lovgren A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-NOV-2013) Astrazeneca R&D Molndal, Pepparedsleden 1, 
            Molndal 43183, Sweden
REFERENCE   3  (bases 1 to 5868)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Expression vector pZeoSv2-Furin"
                     /mol_type="other DNA"
                     /db_xref="taxon:1481780"
     promoter        22..351
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      202..337
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        401..419
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             456..2840
                     /codon_start=1
                     /product="furin"
                     /label=furin
                     /protein_id="AHV78698.1"
                     /translation="MELRPWLLWVVAATGTLVLLAADAQGQKVFTNTWAVRIPGGPAVA
                     NSVARKHGFLNLGQIFGDYYHFWHRGVTKRSLSPHRPRHSRLQREPQVQWLEQQVAKRR
                     TKRDVYQEPTDPKFPQQWYLSGVTQRDLNVKAAWAQGYTGHGIVVSILDDGIEKNHPDL
                     AGNYDPGASFDVNDQDPDPQPRYTQMNDNRHGTRCAGEVAAVANNGVCGVGVAYNARIG
                     GVRMLDGEVTDAVEARSLGLNPNHIHIYSASWGPEDDGKTVDGPARLAEEAFFRGVSQG
                     RGGLGSIFVWASGNGGREHDSCNCDGYTNSIYTLSISSATQFGNVPWYSEACSSTLATT
                     YSSGNQNEKQIVTTDLRQKCTESHTGTSASAPLAAGIIALTLEANKNLTWRDMQHLVVQ
                     TSKPAHLNANDWATNGVGRKVSHSYGYGLLDAGAMVALAQNWTTVAPQRKCIIDILTEP
                     KDIGKRLEVRKTVTACLGEPNHITRLEHAQARLTLSYNRRGDLAIHLVSPMGTRSTLLA
                     ARPHDYSADGFNDWAFMTTHSWDEDPSGEWVLEIENTSEANNYGTLTKFTLVLYGTAPE
                     GLPVPPESSGCKTLTSSQACVVCEEGFSLHQKSCVQHCPPGFAPQVLDTHYSTENDVET
                     IRASVCAPCHASCATCQGPALTDCLSCPSHASLDPVEQTCSRQSQSSRESPPQQQPPRL
                     PPEVEAGQRLRAGLLPSHLPEVVAGLSCAFIVLVFVTVFLVLQLRSGFSFRGVKVYTMD
                     RGLISYKGLPPEAWQEECPSDSEEDEGRGERTAFIKDQSAL"
     regulatory      1753..1762
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     polyA_signal    2919..3143
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(3226..3814)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      4169..4624
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     enhancer        4660..4963
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4964..5167
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        5285..5332
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5351..5725
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5739..5
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"