LOCUS       Exported                5932 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pZ1918 with lacZ cassette, complete sequence.
ACCESSION   U03993
VERSION     .
KEYWORDS    beta-lactamase; beta-galactosidase.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Schweizer HP.
  TITLE     Two plasmids, X1918 and Z1918, for easy recovery of the xylE and 
            lacZ reporter genes
  JOURNAL   Gene 134 (1), 89-91 (1993)
  PUBMED    8244036
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   Su TZ, Schweizer H, Oxender DL.
  TITLE     A novel phosphate-regulated expression vector in Escherichia coli
  JOURNAL   Gene 90 (1), 129-133 (1990)
  PUBMED    2379833
REFERENCE   3  (bases 1 to 5932)
  AUTHORS   Simons RW, Houman F, Kleckner N.
  TITLE     Improved single and multicopy lac-based cloning vectors for protein 
            and operon fusions
  JOURNAL   Gene 53 (1), 85-96 (1987)
  PUBMED    3596251
REFERENCE   4  (bases 1 to 5932)
  AUTHORS   Casadaban MJ, Martinez-Arias A, Shapira SK, Chou J.
  TITLE     Beta-galactosidase gene fusions for analyzing gene expression in 
            escherichia coli and yeast
  JOURNAL   Meth. Enzymol. 100, 293-308 (1983)
  PUBMED    6312261
REFERENCE   5  (bases 1 to 5932)
  AUTHORS   Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-1993) Herbert P. Schweizer, Microbiology and 
            Infectious Diseases, University of Calgary, 3330 Hospital Drive 
            N.W., Calgary, Alberta, T2N 4N1, Canada
REFERENCE   6  (bases 1 to 5932)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Cloning vector pZ1918"
                     /plasmid="pZ1918"
                     /lab_host="Escherichia coli"
                     /mol_type="unassigned DNA"
                     /note="nucleotides 1-449, 3648-5932 from vector pUC1918; 
                     450-3647 from plasmid pTZ1"
                     /db_xref="taxon:31867"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    399..455
                     /standard_name="polylinker"
                     /label=polylinker
     misc_feature    450..3647
                     /label=fragment from pTZ1
                     /note="fragment from pTZ1"
                     /citation="[2]"
     misc_feature    458..532
                     /label=Escherichia coli trp operon DNA
                     /note="Escherichia coli trp operon DNA"
                     /citation="[3]"
     misc_feature    533..3639
                     /label=Escherichia coli lac operon DNA
                     /note="Escherichia coli lac operon DNA"
     primer_bind     538..554
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            539..3624
                     /gene="lacZ"
                     /label=lacZ
     regulatory      539..542
                     /regulatory_class="ribosome_binding_site"
                     /gene="lacZ"
     CDS             550..3624
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /protein_id="AAC53604.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(570..586)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3619..3647
                     /label=junction between lac operon DNA and polylinker
                     /note="junction between lac operon DNA and polylinker"
                     /citation="[4]"
     misc_feature    3642..3698
                     /standard_name="polylinker"
                     /label=polylinker
     primer_bind     complement(3711..3727)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3735..3751
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3759..3789)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3804..3825
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4113..4701)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     gene            4872..5741
                     /gene="bla"
                     /label=bla
     CDS             complement(4872..5732)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AAC53605.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5733..5837)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      5738..5741
                     /regulatory_class="ribosome_binding_site"
                     /gene="bla"