LOCUS       Exported                9215 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pYZ1N, complete sequence.
ACCESSION   AF049063
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   Zhao Y, Chen M, Elder RT, Cao J.
  TITLE     Fission yeast expression vectors adapted for large scale random 
            cloning and GFP fusion with positive selection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9215)
  AUTHORS   Zhao Y, Chen M, Elder RT, Cao J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-1998) Departments of Pediatrics and 
            Microbiology-Immunology, Northwestern University Medical School, 
            2430 N Halsted Street, #218, Chicago, IL 60614, USA
REFERENCE   3  (bases 1 to 9215)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9215
                     /organism="Expression vector pYZ1N"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:73424"
     regulatory      17..1210
                     /regulatory_class="promoter"
                     /note="from nmt1"
     regulatory      1108..1116
                     /regulatory_class="TATA_box"
     CDS             complement(1205..1447)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWHMI"
     misc_feature    complement(1211..1656)
                     /label=Escherichia coli lac operon
                     /note="Escherichia coli lac operon"
     CDS             complement(1211..1447)
                     /codon_start=1
                     /product="lacZ alpha peptide"
                     /label=lacZ alpha peptide
                     /protein_id="AAC78771.1"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWHM"
     primer_bind     1357..1373
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1374..1430
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1443..1459)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1467..1483
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1491..1521)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1536..1557
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     regulatory      1656..2663
                     /regulatory_class="terminator"
                     /note="from nmt1"
     rep_origin      2663..3863
                     /label=from fission yeast ars1
                     /note="from fission yeast ars1"
     rep_origin      2736..3519
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     misc_feature    3864..6973
                     /note="pUC119 sequence; contains ampicillin resistance 
                     gene"
     primer_bind     complement(3869..3885)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4098..4553
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4835..4939
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4940..5800
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AAC78772.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            4940..5800
                     /gene="bla"
                     /label=bla
                     /note="ampicillin resistance gene"
     rep_origin      5971..6559
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6847..6868
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6883..6913
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6921..6937
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6945..6961
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6974..9215
                     /note="Saccharomyces cerevisiae leucine biosynthesis gene 
                     LEU2"
     CDS             complement(7462..8556)
                     /codon_start=1
                     /gene="Leu2"
                     /product="beta-isopropylmalate dehydrogenase"
                     /function="involved in leucine biosynthesis"
                     /label=Leu2
                     /protein_id="AAC78773.1"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     gene            complement(7462..8556)
                     /gene="Leu2"
                     /label=Leu2
     promoter        complement(8569..8973)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter