LOCUS       Exported                4921 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pYUB28b, complete sequence.
ACCESSION   HQ247814
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Bashiri G, Rehan AM, Greenwood DR, Dickson JM, Baker EN.
  TITLE     Metabolic Engineering of Cofactor F(420) Production in Mycobacterium
            smegmatis
  JOURNAL   PLoS ONE 5 (12), E15803 (2010)
  PUBMED    21209917
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Bashiri G, Baker EN.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2010) School of Biological Sciences, The 
            University of Auckland, 3a Symonds St., Auckland, Auckland 1010, New
            Zealand
REFERENCE   3  (bases 1 to 4921)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Cloning vector pYUB28b"
                     /mol_type="other DNA"
                     /label=E. coli/Mycobacterium shuttle vector
                     /note="E. coli/Mycobacterium shuttle vector"
                     /db_xref="taxon:944548"
     misc_feature    1..4921
                     /note="contains hygromycin B resistance protein, OriE (E. 
                     coli origin of replication), OriM (Mycobacterial origin of 
                     replication), T7 promoter, T7 terminator, and multiple 
                     cloning site"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    80..104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             119..141
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             160..177
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             187..204
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             208..240
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             289..306
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      373..420
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      457..885
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      complement(3001..3589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"