LOCUS       Exported               11784 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pYT923hyg DNA, complete sequence.
ACCESSION   AB513495
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11784)
  AUTHORS   Nomoto M, Ogawa M, Fukuda K, Miyamoto H, Taniguchi H.
  TITLE     A host-vector system for molecular study of the intracellular growth
            of Mycobacterium tuberculosis in phagocytic cells
  JOURNAL   Microbiol. Immunol. 53 (10), 550-558 (2009)
  PUBMED    19780968
REFERENCE   2  (bases 1 to 11784)
  AUTHORS   Fukuda K, Taniguchi H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2009) Contact:Kazumasa Fukuda University of 
            Occupational and Environmental Health, JAPAN, Department of 
            Microbiology, School of Medicine; 1-1 ISEIGAOKA,YAHATANISHI-KU, 
            KITAKYUSHU, FUKUOKA 807-8555, Japan
REFERENCE   3  (bases 1 to 11784)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11784
                     /organism="Cloning vector pYT923hyg"
                     /mol_type="other DNA"
                     /db_xref="taxon:661031"
     rep_origin      2..2505
                     /label=replication region in BCG
                     /note="replication region in BCG"
     CDS             complement(4690..5505)
                     /codon_start=1
                     /product="hypothetical protein"
                     /function="kanamaycin resistance"
                     /label=hypothetical protein
                     /protein_id="BAH86059.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(6100..6645)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(6810..7670)
                     /codon_start=1
                     /gene="Amp"
                     /product="beta-lactamase"
                     /label=Amp
                     /protein_id="BAH86060.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(6810..7670)
                     /gene="Amp"
                     /label=Amp
     promoter        complement(7671..7775)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(8593..9591)
                     /codon_start=1
                     /product="hypothetical protein"
                     /function="hygromycin resistance"
                     /label=hypothetical protein
                     /protein_id="BAH86061.1"
                     /translation="MTQESLLLLDRIDSDDSYASLRNDQEFWEPLARRALEELGLPVPP
                     VLRVPGESTNPVLVGEPGPVIKLFGEHWCGPESLASESEAYAVLADAPVPVPRLLGRGE
                     LRPGTGAWPWPYLVMSRMTGTTWRSAMDGTTDRNALLALARELGRVLGRLHRVPLTGNT
                     VLTPHSEVFPELLRERRAATVEDHRGWGYLSPRLLDRLEDWLPDVDTLLAGREPRFVHG
                     DLHGTNIFVDLAATEVTGIVDFTDVYAGDSRYSLVQLHLNAFRGDREILAALLDGAQWK
                     RTEDFARELLAFTFLHDFEVFEETPLDLSGFTDPEELAQFLWGPPDTAPGA"
     primer_bind     complement(9797..9813)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9821..9837
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9845..9875)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9890..9911
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    10381..10521
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10707..11295)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"