LOCUS       Exported               11783 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Gene deletion vector pYS1, complete sequence.
ACCESSION   KF582040
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11783)
  AUTHORS   Shenkerman Y, Elharar Y, Vishkautzan M, Gur E.
  TITLE     Efficient and simple generation of unmarked gene deletions in 
            Mycobacterium smegmatis
  JOURNAL   Gene 533 (1), 374-378 (2014)
  PUBMED    24100088
REFERENCE   2  (bases 1 to 11783)
  AUTHORS   Shenkerman Y, Elharar Y, Vishkautzan M, Gur E.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2013) Life Sciences, Ben-Gurion University of the 
            Negev, Rager Blvd, Beer-Sheva 84105, Israel
REFERENCE   3  (bases 1 to 11783)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11783
                     /organism="Gene deletion vector pYS1"
                     /lab_host="Mycobacterium smegmatis"
                     /mol_type="other DNA"
                     /db_xref="taxon:1408815"
     promoter        216..648
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     misc_feature    286..632
                     /label=sac regulatory region
                     /note="sac regulatory region"
     CDS             649..2109
                     /codon_start=1
                     /gene="sacB"
                     /product="SacB"
                     /label=sacB
                     /note="derived from B.subtilis levansucrase"
                     /protein_id="AGY30912.1"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEAAARCARSFGCGERYQLTQRR"
     gene            649..2109
                     /gene="sacB"
                     /label=sacB
     rep_origin      2181..2848
                     /label=ColE1 origin
                     /note="ColE1 origin"
     rep_origin      complement(2210..2798)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2964..5717
                     /label=mycobacterial PAL5000ts replication origin
                     /note="mycobacterial PAL5000ts replication origin"
     CDS             complement(5953..6768)
                     /codon_start=1
                     /gene="aph"
                     /product="Aph"
                     /label=aph
                     /note="kanamycin resistance"
                     /protein_id="AGY30911.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(5953..6768)
                     /gene="aph"
                     /label=aph
     terminator      6929..6975
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(7040..8101)
                     /codon_start=1
                     /gene="che9c 61"
                     /product="Che9c 61"
                     /label=che9c 61
                     /protein_id="AGY30910.1"
                     /translation="MAENAVTKQDSPKAPETISQVLQVLVPQLARAVPKGMDPDRIARI
                     VQTEIRKSRNAKAAGIAKQSLDDCTQESFAGALLTSAALGLEPGVNGECYLVPYRDTRR
                     GVVECQLIIGYQGIVKLFWQHPRASRIDAQWVGANDEFHYTMGLNPTLKHVKAKGDRGN
                     PVYFYAIVEVTGAEPLWDVFTADEIRELRRGKVGSSGDIKDPQRWMERKTALKQVLKLA
                     PKTTRLDAAIRADDRPGTDLSQSQALALPSTVKPTADYIDGEIAEPHEVDTPPKSSRAQ
                     RAQRATAPAPDVQMANPDQLKRLGEIQKAEKYNDADWFKFLADSAGVKATRAADLTFDE
                     AKAVIDMFDGPNA"
     gene            complement(7040..8101)
                     /gene="che9c 61"
                     /label=che9c 61
     CDS             complement(8094..9038)
                     /codon_start=1
                     /gene="che9c 60"
                     /product="Che9c 60"
                     /label=che9c 60
                     /protein_id="AGY30909.1"
                     /translation="MSVPTQDGMHRFVDEDVYHADRGSLSVSGAKLLLPPSCPAKFRWE
                     MDNTRKPKKVWDFGHVAHKLVLGKGAEFEILDPEVHGLKADGTPSEKPTATGMWRKAEA
                     EARKQGKVPIHVDLFTKAYDMAEKVRQHPTAGPIFANPDGEAEVALYYTDPETGVRLRG
                     RIDWLTDDIDDYKTSMTANPAELKTKFYKLGYFMQAAWYIDLLVALGLAENPRFRFITQ
                     EKEPPYVVTPIQYDDEAIEEGRRRNRQAIRLYADCMESGKWPDYSDDVVTISLPSWGLP
                     RPQTVGDVVTDSYIYDTDPLEEADPIEGDYIYG"
     gene            complement(8094..9038)
                     /gene="che9c 60"
                     /label=che9c 60
     CDS             complement(9127..9768)
                     /codon_start=1
                     /gene="amiS"
                     /product="AmiS acetamidase regulator"
                     /label=amiS
                     /protein_id="AGY30908.1"
                     /translation="MGGVGLFYVGAVLIIDGLMLLGRISPRGATPLNFFVGGLQVVTPT
                     VLILQSGGDAAVIFAASGLYLFGFTYLWVAINNVTDWDGEGLGWFSLFVAIAALGYSWH
                     AFTAEADPAFGVIWLLWAVLWFMLFLLLGLGHDALGPAVGFVAVAEGVITAAVPAFLIV
                     SGNWETGPLPAAVIAVIGFAAVVLAYPIGRRLAAPSVTNPPPAALAATTR"
     gene            complement(9127..9768)
                     /gene="amiS"
                     /label=amiS
     CDS             complement(9773..10057)
                     /codon_start=1
                     /gene="amiD"
                     /product="AmiD acetamidase regulator"
                     /label=amiD
                     /protein_id="AGY30907.1"
                     /translation="MPTYTFRCSHCGPFDLTCAISERDAAATCPECRTPARRVFGSVGL
                     TTFTAGHHRAFDAASASAESPTVVKSIPAGADRPRAPRRNPGLPSLPRY"
     gene            complement(9773..10057)
                     /gene="amiD"
                     /label=amiD
     CDS             complement(10137..10565)
                     /codon_start=1
                     /gene="amiA"
                     /product="AmiA acetamidase regulator"
                     /label=amiA
                     /protein_id="AGY30906.1"
                     /translation="MAAGQQRRPNLLLPLVRLTHLAESAIERVLADSSLKIEDWRVLDE
                     LAGRRTVPMSDLAQATLITGPTLTRTVDRLVSQGIIYRTADLHDRRRVLVALTPRGRTL
                     RNRLVDAVAEAECAAFESCGLDVDQLRELVDTTSNLTS"
     gene            complement(10137..10565)
                     /gene="amiA"
                     /label=amiA
     CDS             10668..11741
                     /codon_start=1
                     /gene="amiC"
                     /product="AmiC acetamidase regulator"
                     /label=amiC
                     /protein_id="AGY30905.1"
                     /translation="MQDGEVEFRVGLVIPLQGPAGIFAPSCEAVAELAAKEVNDRGGLQ
                     GRKVTIEVLDGGRPGDDVARTVADRLRGHGLDAVTGWHISAVRNRISPVVRDRIPYVYT
                     SLYEGGERTPGVFCTGETPQIQIAPALAWLRDHFGIRSWCLVGDDYIWPRRSAAAARAY
                     CRDLDLELRREIYVPYGTDDFRAPVRKAIASGAQAVLMLLVGQDAVLFNREFARAGGHD
                     RMARFSPLMEENMLLASGAGSTENLYVAAAYFSSLATAGAMDLMGSYVARYGADAPPLN
                     AMAESCYEGLVALEAIFQRAHSPEIPDLMASAHDVGFDGPRGPMCMRDSQFDQQVYIAS
                     ADGYDFDILDTLTTLDA"
     gene            10668..11741
                     /gene="amiC"
                     /label=amiC