LOCUS       Exported                4641 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Suicide vector pYMD1 DNA, complete sequence.
ACCESSION   AB304880
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Yamada K, Kaneko J, Kamio Y, Itoh Y.
  TITLE     Binding sequences for RdgB, a DNA damage-responsive transcriptional 
            activator, and temperature-dependent expression of bacteriocin and 
            pectin lyase genes in Pectobacterium carotovorum subsp. carotovorum
  JOURNAL   Appl. Environ. Microbiol. 74 (19), 6017-6025 (2008)
  PUBMED    18689515
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Yamada K, Kaneko J, Kamio Y, Itoh Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2007) Contact:Kazuteru Yamada Graduate School of 
            Agricultural Science, Tohoku University, Laboratory of Applied 
            Microbiology, Department of Microbial Biotechnology; 1-1 
            Tsutsumi-dori Amaiya-machi, Aobaku, Sendai 981-8555, Japan
REFERENCE   3  (bases 1 to 4641)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Suicide vector pYMD1"
                     /mol_type="other DNA"
                     /db_xref="taxon:445436"
     primer_bind     5..21
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        110..555
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             556..1977
                     /codon_start=1
                     /gene="sacB"
                     /product="levansucrase"
                     /label=sacB
                     /protein_id="BAF93887.1"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     gene            556..1977
                     /gene="sacB"
                     /label=sacB
     primer_bind     complement(2708..2724)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2760..3116
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(3649..4509)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="BAF93888.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3649..4509)
                     /gene="bla"
                     /label=bla
     promoter        complement(4510..4614)
                     /gene="bla"
                     /label=AmpR promoter