LOCUS       Exported                7331 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pYM70, complete sequence.
ACCESSION   GU937092
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7331)
  AUTHORS   Basso LR Jr., Gast CE, Mao Y, Wong B.
  TITLE     Fluconazole transport into Candida albicans secretory vesicles by 
            the membrane proteins Cdr1p, Cdr2p, and Mdr1p
  JOURNAL   Eukaryotic Cell 9 (6), 960-970 (2010)
  PUBMED    20348384
REFERENCE   2  (bases 1 to 7331)
  AUTHORS   Basso LR Jr., Bartiss A, Mao Y, Gast CE, Coelho PS, Snyder M, Wong 
            B.
  TITLE     Transformation of Candida albicans with a synthetic hygromycin B 
            resistance gene
  JOURNAL   Yeast 27 (12), 1039-1048 (2010)
  PUBMED    20737428
REFERENCE   3  (bases 1 to 7331)
  AUTHORS   Mao Y, Basso LR Jr., Gast CE, Wong B.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2010) Medicine, Oregon Health and Science 
            University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
REFERENCE   4  (bases 1 to 7331)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7331
                     /organism="Expression vector pYM70"
                     /lab_host="Candida albicans"
                     /mol_type="other DNA"
                     /db_xref="taxon:754152"
     rep_origin      189..1390
                     /label=Candida albicans autonomous replication origin 2
                     /note="Candida albicans autonomous replication origin 2"
     primer_bind     1587..1603
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      1637..2652
                     /regulatory_class="promoter"
                     /note="Candida albicans ACT1 promoter"
     regulatory      2684..3009
                     /regulatory_class="terminator"
                     /note="Candida albicans TEF2 terminator"
     primer_bind     complement(3037..3053)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3061..3077
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3085..3115)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3130..3151
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3424..4043)
                     /direction=LEFT
                     /label=Escherichia coli replication origin pMB1
                     /note="Escherichia coli replication origin pMB1"
     rep_origin      complement(3439..4027)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4198..5058)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="derived from Escherichia coli"
                     /protein_id="ADF45589.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(4198..5058)
                     /gene="bla"
                     /label=bla
     promoter        complement(5059..5163)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      5195..5835
                     /regulatory_class="promoter"
                     /note="Candida albicans TEF2 promoter"
     CDS             5842..6867
                     /codon_start=1
                     /gene="CaHygB"
                     /product="hygromycin B phosphotransferase"
                     /label=CaHygB
                     /note="synthetic; optimized for expression in Candida 
                     albicans"
                     /protein_id="ADF45590.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     gene            5842..6867
                     /gene="CaHygB"
                     /label=CaHygB
     regulatory      6874..7261
                     /regulatory_class="terminator"
                     /note="Candida albicans ACT1 terminator"