LOCUS       Exported                8528 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pYGKlacZ, complete sequence.
ACCESSION   JX826405
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8528)
  AUTHORS   Juarez-Rodriguez MD, Torres-Escobar A, Demuth DR.
  TITLE     Construction of new cloning, lacZ reporter and scarless-markerless 
            suicide vectors for genetic studies in Aggregatibacter 
            actinomycetemcomitans
  JOURNAL   Plasmid 69 (3), 211-222 (2013)
  PUBMED    23353051
REFERENCE   2  (bases 1 to 8528)
  AUTHORS   Juarez-Rodriguez MD, Torres-Escobar A, Demuth DR.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2012) Research Group in Oral Health and Systemic 
            Disease, University of Louisville School of Dentistry, 501 S. 
            Preston Street, Louisville, KY 40202, USA
REFERENCE   3  (bases 1 to 8528)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8528
                     /organism="Shuttle vector pYGKlacZ"
                     /mol_type="other DNA"
                     /note="clinical isolate from case of swine 
                     pleuropneumoniae; derived from Actinobacillus 
                     pleuropneumoniae 80-8141 pYG10"
                     /db_xref="taxon:1260098"
     CDS             32..3091
                     /codon_start=1
                     /gene="lacZ"
                     /product="B-galactosidase"
                     /label=lacZ
                     /note="derived from Escherichia coli strain C600"
                     /protein_id="AFX62587.1"
                     /translation="MITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDR
                     PSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTY
                     PITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQD
                     SRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQIS
                     DFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDER
                     GGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGL
                     LLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYT
                     LCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLG
                     NESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKW
                     SIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIK
                     YDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTI
                     EVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLW
                     LTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNK
                     RWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAA
                     GHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEV
                     ASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVF
                     PSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFH
                     MGIGGDDSWSPSVSAEFQLSAGRYHYQLVW"
     gene            32..3091
                     /gene="lacZ"
                     /label=lacZ
     primer_bind     complement(46..62)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3257..4387)
                     /codon_start=1
                     /gene="mobA"
                     /product="MobA"
                     /label=mobA
                     /note="involved in plasmid mobilization"
                     /protein_id="AFX62588.1"
                     /translation="MIVKFFKKHGKGKASSCKACVDYLLNKPDDTAQILQGDPRLSQSI
                     ADSLDFNNTYTAGCLSFEESDLPEIQKREIMARFEKAMFAGLEPEQYNIAWVQHTDKGR
                     LELNFVIPNVEMTSGKRLQPYYDRADRPLAENFKQVINHEYSLSDPNNPIKQKNLIDRK
                     DLPTDKKQALQAITDGLTALANAGQINDRQDVINALERAGFEIARITPKNLSIKTDGQN
                     LRLKGAFYEQDFRFSTDLSADITERAREYKRDSAERYQTARAKLDTAVTARREQFSRKY
                     PNRAGEIDKKYRENVSLADPNRLDDINLDSHNHSISGSLISCLKILVNLSNFVHLLKVL
                     KTVLYFPKHSGKYRHGQPVFRRYKIPVKIFLKFGLS"
     gene            complement(3257..4387)
                     /gene="mobA"
                     /label=mobA
     CDS             complement(3427..3699)
                     /codon_start=1
                     /gene="mobB"
                     /product="MobB"
                     /label=mobB
                     /note="involved in plasmid mobilization"
                     /protein_id="AFX62589.1"
                     /translation="MSKILDLAQTFQQTSQKELESTSEIVQNAIKRHEQNLIQQLQHAG
                     NNLAENIQIEQAKLIKNTVRMYRLPTLIALTILTLIAITTPYPDL"
     gene            complement(3427..3699)
                     /gene="mobB"
                     /label=mobB
     CDS             complement(4384..4689)
                     /codon_start=1
                     /gene="mobC"
                     /product="MobC"
                     /label=mobC
                     /note="involved in plasmid mobilization"
                     /protein_id="AFX62590.1"
                     /translation="MKREKEIKIRLTENEYQALLERKTKARLAEWVREVALEQQPKRQP
                     KVIDPALLFELNRIGVNLNQIARQCNSQKPSIDLVSVLATLREIEKNLKKLRELSL"
     gene            complement(4384..4689)
                     /gene="mobC"
                     /label=mobC
     CDS             complement(4762..5022)
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="orf2"
                     /protein_id="AFX62591.1"
                     /translation="MDNSASYPRAFQQLSNTKNLPPTITTSLIIKFFYFYFGSKAHDVR
                     LIKRSRLSAPLIFIYHSRGFWCTIVFCNSKDTKRVLFEFPF"
     repeat_region   4973..5047
     repeat_region   4974..4996
     repeat_region   5025..5040
     repeat_region   5035..5041
     repeat_region   5097..5112
     repeat_region   5273..5279
     CDS             complement(5335..5454)
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="orf1"
                     /protein_id="AFX62592.1"
                     /translation="MQEIAKRPYYQKTIQRRKQTNANLKLDGLQLGLRKNKQA"
     repeat_region   5466..5509
     repeat_region   5528..5569
     CDS             complement(5572..5688)
                     /codon_start=1
                     /gene="strB"
                     /product="truncated aminoglycoside 6-phosphotranferase"
                     /label=strB
                     /note="APH0(6)-id; streptomycin resistance protein"
                     /protein_id="AFX62594.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPVIPML"
     gene            complement(5572..5688)
                     /gene="strB"
                     /label=strB
     CDS             complement(5688..6491)
                     /codon_start=1
                     /gene="strA"
                     /product="aminoglycoside 3-phosphotranferase"
                     /label=strA
                     /note="APH0(3)-Ib"
                     /protein_id="AFX62593.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRR
                     GELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPS
                     MVQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARV
                     ERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIA
                     NAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(5688..6491)
                     /gene="strA"
                     /label=strA
     misc_feature    complement(6560..7096)
                     /note="similar to chloramphenicol acetyltransferase type 
                     III"
     CDS             7229..8044
                     /codon_start=1
                     /gene="aph(3)-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=aph(3)-Ia
                     /note="kanamycin resistance protein from pUC4K"
                     /protein_id="AFX62595.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            7229..8044
                     /gene="aph(3)-Ia"
                     /label=aph(3)-Ia
     misc_feature    complement(8353..8457)
                     /note="similar to chloramphenicol acetyltransferase type 
                     III"