LOCUS       Exported                5367 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pYGK, complete sequence.
ACCESSION   JX826404
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Juarez-Rodriguez MD, Torres-Escobar A, Demuth DR.
  TITLE     Construction of new cloning, lacZ reporter and scarless-markerless 
            suicide vectors for genetic studies in Aggregatibacter 
            actinomycetemcomitans
  JOURNAL   Plasmid 69 (3), 211-222 (2013)
  PUBMED    23353051
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   Juarez-Rodriguez MD, Torres-Escobar A, Demuth DR.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2012) Research Group in Oral Health and Systemic 
            Disease, University of Louisville School of Dentistry, 501 S. 
            Preston Street, Louisville, KY 40202, USA
REFERENCE   3  (bases 1 to 5367)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Shuttle vector pYGK"
                     /mol_type="other DNA"
                     /note="clinical isolate from case of swine pleuropneumonia;
                     derived from Actinobacillus pleuropneumoniae 80-8141 pYG10"
                     /db_xref="taxon:1260097"
     misc_feature    1..57
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             complement(96..1226)
                     /codon_start=1
                     /gene="mobA"
                     /product="MobA"
                     /label=mobA
                     /note="involved in plasmid mobilization"
                     /protein_id="AFX62584.1"
                     /translation="MIVKFFKKHGKGKASSCKACVDYLLNKPDDTAQILQGDPRLSQSI
                     ADSLDFNNTYTAGCLSFEESDLPEIQKREIMARFEKAMFAGLEPEQYNIAWVQHTDKGR
                     LELNFVIPNVEMTSGKRLQPYYDRADRPLAENFKQVINHEYSLSDPNNPIKQKNLIDRK
                     DLPTDKKQALQAITDGLTALANAGQINDRQDVINALERAGFEIARITPKNLSIKTDGQN
                     LRLKGAFYEQDFRFSTDLSADITERAREYKRDSAERYQTARAKLDTAVTARREQFSRKY
                     PNRAGEIDKKYRENVSLADPNRLDDINLDSHNHSISGSLISCLKILVNLSNFVHLLKVL
                     KTVLYFPKHSGKYRHGQPVFRRYKIPVKIFLKFGLS"
     gene            complement(96..1226)
                     /gene="mobA"
                     /label=mobA
     CDS             complement(266..538)
                     /codon_start=1
                     /gene="mobB"
                     /product="MobB"
                     /label=mobB
                     /note="involved in plasmid mobilization"
                     /protein_id="AFX62585.1"
                     /translation="MSKILDLAQTFQQTSQKELESTSEIVQNAIKRHEQNLIQQLQHAG
                     NNLAENIQIEQAKLIKNTVRMYRLPTLIALTILTLIAITTPYPDL"
     gene            complement(266..538)
                     /gene="mobB"
                     /label=mobB
     CDS             complement(1223..1528)
                     /codon_start=1
                     /gene="mobC"
                     /product="MobC"
                     /label=mobC
                     /note="involved in plasmid mobilization"
                     /protein_id="AFX62586.1"
                     /translation="MKREKEIKIRLTENEYQALLERKTKARLAEWVREVALEQQPKRQP
                     KVIDPALLFELNRIGVNLNQIARQCNSQKPSIDLVSVLATLREIEKNLKKLRELSL"
     gene            complement(1223..1528)
                     /gene="mobC"
                     /label=mobC
     CDS             complement(1601..1861)
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="orf2"
                     /protein_id="AFX62579.1"
                     /translation="MDNSASYPRAFQQLSNTKNLPPTITTSLIIKFFYFYFGSKAHDVR
                     LIKRSRLSAPLIFIYHSRGFWCTIVFCNSKDTKRVLFEFPF"
     repeat_region   1812..1886
     repeat_region   1813..1835
     repeat_region   1864..1879
     repeat_region   1936..2951
     repeat_region   2102..2108
     CDS             complement(2174..2293)
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="orf1"
                     /protein_id="AFX62580.1"
                     /translation="MQEIAKRPYYQKTIQRRKQTNANLKLDGLQLGLRKNKQA"
     repeat_region   2305..2348
     repeat_region   2368..2408
     CDS             complement(2411..2521)
                     /codon_start=1
                     /gene="strB"
                     /product="aminoglycoside 6-phosphotranferase (APH0(6)-id)"
                     /label=strB
                     /protein_id="AFX62581.1"
                     /translation="MPPVFPAHWHVSQPVLIADTFSSLVWKVSLPVIPML"
     gene            complement(2411..2521)
                     /gene="strB"
                     /label=strB
     CDS             complement(2527..3330)
                     /codon_start=1
                     /gene="strA"
                     /product="aminoglycoside 3-phosphotranferase"
                     /label=strA
                     /note="APH0(3)-Ib"
                     /protein_id="AFX62582.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRR
                     GELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPS
                     MVQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARV
                     ERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIA
                     NAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(2527..3330)
                     /gene="strA"
                     /label=strA
     misc_feature    complement(3399..3935)
                     /note="similar to chloramphenicol acetyltransferase type 
                     III"
     CDS             4068..4883
                     /codon_start=1
                     /gene="aph(3)-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=aph(3)-Ia
                     /protein_id="AFX62583.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            4068..4883
                     /gene="aph(3)-Ia"
                     /label=aph(3)-Ia
                     /note="aph(3)-Ia encodes kanamycin resistance marker from 
                     pUC4K cloning vector"
     misc_feature    complement(5192..5296)
                     /note="similar to chloramphenicol acetyltransferase type 
                     III"