LOCUS       Exported                7410 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Integration vector pYFP_Star plasmid pYFP_Star, complete sequence.
ACCESSION   KJ411637
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Trauth S, Bischofs IB.
  TITLE     Ectopic Integration Vectors for Generating Fluorescent Promoter 
            Fusions in Bacillus subtilis with Minimal Dark Noise
  JOURNAL   PLoS ONE 9 (5), E98360 (2014)
  PUBMED    24874808
REFERENCE   2  (bases 1 to 7410)
  AUTHORS   Trauth S, Bischofs IB.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2014) Center for Molecular Biology and BioQuant, 
            University of Heidelberg, Im Neuenheimer Feld 282, Heidelberg, BW 
            69120, Germany
REFERENCE   3  (bases 1 to 7410)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="Integration vector pYFP_Star"
                     /plasmid="pYFP_Star"
                     /mol_type="other DNA"
                     /db_xref="taxon:1506529"
     promoter        104..208
                     /gene="bla"
                     /label=AmpR promoter
     CDS             209..1069
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampicillin resistance determinant"
                     /protein_id="AIC79142.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            209..1069
                     /gene="bla"
                     /label=bla
     misc_feature    1231..2545
                     /label=ColE1 region involved in plasmid replication
                     /note="ColE1 region involved in plasmid replication"
     rep_origin      1240..1828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      1829
                     /label=ColE1 origin of DNA replication (+1)
                     /note="ColE1 origin of DNA replication (+1)"
     misc_feature    2014..2154
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2256..2447)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_recomb     complement(3384..4579)
                     /label=homologous recombination site amyE back
                     /note="homologous recombination site amyE back"
     CDS             complement(4949..5599)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /note="chloramphenicol resistance determinant"
                     /protein_id="AIC79143.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     gene            complement(4949..5599)
                     /gene="cat"
                     /label=cat
     misc_feature    5763..5779
                     /label=small cloning site (SCS)
                     /note="small cloning site (SCS)"
     regulatory      5780..5840
                     /regulatory_class="terminator"
                     /note="Bacillus subtilis gyrA transcriptional terminator"
     stem_loop       5792..5812
                     /label=Bacillus subtilis gyrA transcriptional terminator
                     /note="Bacillus subtilis gyrA transcriptional terminator"
     misc_feature    5845..5873
                     /label=LIC site
                     /note="LIC site"
     CDS             5877..6632
                     /codon_start=1
                     /gene="iyfp"
                     /product="iYFP"
                     /label=iyfp
                     /protein_id="AIC79144.1"
                     /translation="MDSIEKVSEFATMVSKGEELFTGVVPILVELDGDVNGHKFSVSGE
                     GEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEG
                     YVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNV
                     YIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSK
                     DPNEKRDHMVLLEFVTAAGITLGMDELYK"
     gene            5877..6632
                     /gene="iyfp"
                     /label=iyfp
     regulatory      5907..5916
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             5913..6632
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6634..6650)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6683..6701)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6708..6724)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_recomb     complement(6773..7410)
                     /label=homologous recombination site amyE front
                     /note="homologous recombination site amyE front"