LOCUS       Exported                8452 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Neurospora expression vector pYFP, complete sequence.
ACCESSION   EF661030
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8452)
  AUTHORS   Bardiya N, Alexander WG, Perdue TD, Barry EG, Metzenberg RL, Pukkila
            PJ, Shiu PK.
  TITLE     Characterization of interactions between and among components of the
            meiotic silencing by unpaired DNA machinery in Neurospora crassa 
            using bimolecular fluorescence complementation
  JOURNAL   Genetics 178 (1), 593-596 (2008)
  PUBMED    18202398
REFERENCE   2  (bases 1 to 8452)
  AUTHORS   Shiu PKT.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2007) Division of Biological Sciences, University 
            of Missouri-Columbia, 103 Tucker Hall, Columbia, MO 65203, USA
REFERENCE   3  (bases 1 to 8452)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8452
                     /organism="Neurospora expression vector pYFP"
                     /mol_type="other DNA"
                     /db_xref="taxon:469817"
     promoter        363..467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             468..1328
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampicillin resistance"
                     /protein_id="ABV26714.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            468..1328
                     /gene="bla"
                     /label=bla
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2638..2649
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4008..4026
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4059..4980
                     /regulatory_class="promoter"
                     /note="Neurospora crassa ccg-1 promoter"
     primer_bind     4981..4997
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5028..5747
                     /codon_start=1
                     /gene="yfp"
                     /product="yellow fluorescent protein"
                     /label=yfp
                     /protein_id="ABV26713.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     gene            5028..5747
                     /gene="yfp"
                     /label=yfp
     promoter        complement(5772..5790)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(8451..17)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"