LOCUS       Exported                7133 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pYEULCBX, complete sequence.
ACCESSION   L25833
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7133)
  AUTHORS   Ward AC, Azad AA, Macreadie IG.
  TITLE     Expression and characterisation of the influenza A virus 
            non-structural protein NS1 in yeast
  JOURNAL   Arch. Virol. 138 (3-4), 299-314 (1994)
  PUBMED    7998836
REFERENCE   2  (bases 1 to 7133)
  AUTHORS   Ward AC.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-1993) Alister C. Ward, Medicine, University of 
            Melbourne, Royal Melbourne Hospital, Parkville, Vic 3050, Australia
REFERENCE   3  (bases 1 to 7133)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7133
                     /organism="Cloning vector pYEULCBX"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:39055"
     rep_origin      4..1350
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(1461..2264)
                     /codon_start=1
                     /gene="URA3"
                     /product="OMP decarboxylase"
                     /label=URA3
                     /protein_id="AAA74940.1"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     gene            complement(1461..2264)
                     /gene="URA3"
                     /label=URA3
     promoter        complement(2265..2480)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     promoter        2596..2700
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2701..3561
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="AAA74941.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3732..4320
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4608..4629
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4644..4674
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4682..4698
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4706..4722
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5006..5033
                     /standard_name="MCS"
                     /function="multiple cloning site"
                     /label=MCS
                     /note="putative"
     CDS             complement(5669..6763)
                     /codon_start=1
                     /gene="LEU2"
                     /product="3-isopropylmalate dehydrogenase"
                     /label=LEU2
                     /protein_id="AAA74942.1"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     gene            complement(5669..6763)
                     /gene="LEU2"
                     /label=LEU2