LOCUS       Exported                5123 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pYBA-300, complete sequence.
ACCESSION   KC200570
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Yan X, Wang H, Ye Y, Zeng G, Ma R, Mi F, Yao L.
  TITLE     pYBA100: An Ease-of-use Binary Vector with LoxP-FRT Recombinase Site
            for Plant Transformation
  JOURNAL   Mol. Plant Breed. 10 (3), 371-379 (2012)
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Yao L, Yan X, Wang H, Ye Y, Zeng G, Ma R.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2012) Beijing Agro-Biotechnology Research Center, 
            Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguang 
            Huayuan Zhonglu, Beijing 100097, P.R. China
REFERENCE   3  (bases 1 to 5123)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Binary vector pYBA-300"
                     /mol_type="other DNA"
                     /db_xref="taxon:1301036"
     misc_feature    1..589
                     /note="pUC ori; derived from pUC19"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    594..2367
                     /note="MCS-2; derived from pBBR1"
     rep_origin      741..1510
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1511..2173
                     /codon_start=1
                     /gene="rep"
                     /product="Rep"
                     /label=rep
                     /note="involved in plasmid replication"
                     /protein_id="AGG56505.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     gene            1511..2173
                     /gene="rep"
                     /label=rep
     misc_feature    2368..2391
                     /label=RB overdrive sequence
                     /note="RB overdrive sequence"
     misc_feature    2392..2416
                     /label=T-DNA right border
                     /note="T-DNA right border"
     misc_feature    2417..2649
                     /note="MCS; polylinker; derived from pBluescript"
     misc_feature    2435..2542
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     2452..2468
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2502..2518)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_recomb     2564..2597
                     /label=loxP site
                     /note="loxP site"
     protein_bind    complement(2564..2597)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_recomb     2598..2631
                     /label=FRT site
                     /note="FRT site"
     protein_bind    2598..2631
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    2650..2905
                     /label=NOS terminator
                     /note="NOS terminator"
     terminator      2653..2905
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2906..3457)
                     /codon_start=1
                     /gene="bar"
                     /product="Bar"
                     /label=bar
                     /note="glufosinate (basta herbicide) resistance protein"
                     /protein_id="AGG56506.1"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     gene            complement(2906..3457)
                     /gene="bar"
                     /label=bar
     regulatory      3458..3764
                     /regulatory_class="promoter"
                     /note="NOS promoter"
     promoter        complement(3466..3649)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(3502..3681)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_recomb     3773..3806
                     /label=loxP site
                     /note="loxP site"
     protein_bind    complement(3773..3806)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_recomb     3807..3840
                     /label=FRT site
                     /note="FRT site"
     protein_bind    3807..3840
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    3852..3876
                     /label=T-DNA left border
                     /note="T-DNA left border"
     misc_feature    3879..5123
                     /note="MCS-2; derived from pBBR1"
     CDS             complement(4063..4857)
                     /codon_start=1
                     /gene="nptII"
                     /product="NptII"
                     /label=nptII
                     /note="kanamycin resistance determinant"
                     /protein_id="AGG56507.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            complement(4063..4857)
                     /gene="nptII"
                     /label=nptII