LOCUS       Exported                7458 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pYBA-1351, complete sequence.
ACCESSION   KP150429
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7458)
  AUTHORS   Yao L, Yan X, Wang H, Cao M, Robaglia C.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2014) Beijing Agro-Biotechnology Research Center, 
            Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguang 
            Huayuan Zhonglu, Beijing 100097, P.R. China
REFERENCE   2  (bases 1 to 7458)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7458
                     /organism="Binary vector pYBA-1351"
                     /mol_type="other DNA"
                     /db_xref="taxon:1633527"
     misc_feature    1..589
                     /note="pUC ori; derived from pUC19"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    594..2367
                     /label=derived from pBBR1MCS-2
                     /note="derived from pBBR1MCS-2"
     rep_origin      741..1510
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1511..2173
                     /codon_start=1
                     /gene="rep"
                     /product="Rep"
                     /label=rep
                     /protein_id="AJZ74094.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     gene            1511..2173
                     /gene="rep"
                     /label=rep
                     /note="involved in plasmid replication"
     misc_feature    2368..2391
                     /label=RB overdrive sequence
                     /note="RB overdrive sequence"
     misc_feature    2392..2416
                     /label=T-DNA right border
                     /note="T-DNA right border"
     misc_feature    2428..2628
                     /note="CaMV poly-A signal; derived from pRT103"
     misc_feature    2639..2728
                     /note="MCS; polylinker; derived from pBluescript"
     primer_bind     2662..2678
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2712..2728)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(2729..4537)
                     /gene="gusA"
                     /note="beta-glucuronidase; GUS"
     gene            complement(2729..4537)
                     /gene="gusA"
                     /label=gusA
     CDS             complement(2729..4537)
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     misc_feature    4542..4608
                     /label=TMV omega sequence
                     /note="TMV omega sequence"
     misc_feature    4543..4598
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5-leader sequence (Gallie et al., 1988)"
     regulatory      4609..5028
                     /regulatory_class="promoter"
                     /note="CaMV 35S promoter"
     promoter        complement(4609..4954)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      5053..5308
                     /regulatory_class="terminator"
                     /note="NOS terminator"
     terminator      5056..5308
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5309..5860)
                     /codon_start=1
                     /gene="bar"
                     /product="Bar"
                     /label=bar
                     /note="phosphinothricin acetyltransferase"
                     /protein_id="AJZ74095.1"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     gene            complement(5309..5860)
                     /gene="bar"
                     /label=bar
     regulatory      5861..6167
                     /regulatory_class="promoter"
                     /note="NOS promoter"
     promoter        complement(5869..6052)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(5905..6084)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6187..6211
                     /label=T-DNA left border
                     /note="T-DNA left border"
     misc_feature    6214..7458
                     /label=derived from pBBR1MCS-2
                     /note="derived from pBBR1MCS-2"
     CDS             complement(6398..7192)
                     /codon_start=1
                     /gene="nptII"
                     /product="NptII"
                     /label=nptII
                     /note="kanamycin resistance determinant"
                     /protein_id="AJZ74096.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            complement(6398..7192)
                     /gene="nptII"
                     /label=nptII