LOCUS       Exported                5905 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pYBA-1328, complete sequence.
ACCESSION   KP171159
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Yao L, Yan X, Wang H, Cao M, Robaglia C.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-NOV-2014) Beijing Agro-Biotechnology Research Center, 
            Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguang 
            Huayuan Zhonglu, Beijing, Beijing 100097, P.R. China
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Binary vector pYBA-1328"
                     /mol_type="other DNA"
                     /db_xref="taxon:1630259"
     misc_feature    1..589
                     /note="pUC ori; derived from pUC19"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    594..2367
                     /label=derived from pBBR1MCS-2
                     /note="derived from pBBR1MCS-2"
     rep_origin      741..1510
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1511..2173
                     /codon_start=1
                     /gene="rep"
                     /product="Rep"
                     /label=rep
                     /protein_id="AKA95310.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     gene            1511..2173
                     /gene="rep"
                     /label=rep
                     /note="involved in plasmid replication"
     misc_feature    2368..2391
                     /label=RB overdrive sequence
                     /note="RB overdrive sequence"
     misc_feature    2392..2416
                     /label=T-DNA right border
                     /note="T-DNA right border"
     misc_feature    2428..2628
                     /note="CaMV poly-A signal; derived from pRT103"
     CDS             complement(2636..3352)
                     /codon_start=1
                     /gene="mCherry"
                     /product="mCherry"
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein"
                     /protein_id="AKA95311.1"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKSR"
     gene            complement(2636..3352)
                     /gene="mCherry"
                     /label=mCherry
     CDS             complement(2645..3352)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    3353..3499
                     /note="MCS; polylinker; derived from pBluescript"
     misc_feature    3353..3460
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     3370..3386
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3420..3436)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3500..3755
                     /label=NOS terminator
                     /note="NOS terminator"
     terminator      3503..3755
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3756..4307)
                     /codon_start=1
                     /gene="bar"
                     /product="Bar"
                     /label=bar
                     /note="phosphinothricin acetyltransferase"
                     /protein_id="AKA95312.1"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     gene            complement(3756..4307)
                     /gene="bar"
                     /label=bar
     misc_feature    4308..4614
                     /label=NOS promoter
                     /note="NOS promoter"
     promoter        complement(4316..4499)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(4352..4531)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    4634..4658
                     /label=T-DNA left border
                     /note="T-DNA left border"
     misc_feature    4661..5905
                     /label=derived from pBBR1MCS-2
                     /note="derived from pBBR1MCS-2"
     CDS             complement(4845..5639)
                     /codon_start=1
                     /gene="nptII"
                     /product="NptII"
                     /label=nptII
                     /protein_id="AKA95313.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            complement(4845..5639)
                     /gene="nptII"
                     /label=nptII
                     /note="kanamycin resistance determinant"