LOCUS       Exported                5187 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pYBA-1301, complete sequence.
ACCESSION   KU221177
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Yao L, Yan X, Wang H, Cao M, Robaglia C.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2015) Beijing Agro-Biotechnology Research Center, 
            Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguang 
            Huayuan Zhonglu, Beijing 100097, P.R. China
REFERENCE   2  (bases 1 to 5187)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Binary vector pYBA-1301"
                     /mol_type="other DNA"
                     /db_xref="taxon:1853773"
     rep_origin      1..589
                     /note="pUC ori; derived from pUC19"
     misc_feature    594..2367
                     /label=derived from pBBR1MCS-2
                     /note="derived from pBBR1MCS-2"
     rep_origin      741..1510
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1511..2173
                     /codon_start=1
                     /gene="rep"
                     /product="Rep"
                     /label=rep
                     /note="involved in plasmid replication"
                     /protein_id="ANH21052.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     gene            1511..2173
                     /gene="rep"
                     /label=rep
     misc_feature    2368..2391
                     /label=RB overdrive sequence
                     /note="RB overdrive sequence"
     misc_feature    2392..2416
                     /label=T-DNA right border
                     /note="T-DNA right border"
     regulatory      2428..2628
                     /regulatory_class="polyA_signal_sequence"
                     /note="CaMV poly-A signal; derived from pRT103"
     misc_feature    2629..2781
                     /note="MCS; polylinker; derived from pBluescript"
     misc_feature    2635..2742
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     2652..2668
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2702..2718)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2782..3037
                     /regulatory_class="terminator"
                     /note="NOS terminator"
     terminator      2785..3037
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3038..3589)
                     /codon_start=1
                     /gene="bar"
                     /product="Bar"
                     /label=bar
                     /note="phosphinothricin acetyltransferase"
                     /protein_id="ANH21053.1"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     gene            complement(3038..3589)
                     /gene="bar"
                     /label=bar
     regulatory      3590..3896
                     /regulatory_class="promoter"
                     /note="NOS promoter"
     promoter        complement(3598..3781)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(3634..3813)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    3916..3940
                     /label=T-DNA left border
                     /note="T-DNA left border"
     misc_feature    3943..5187
                     /label=derived from pBBR1MCS-2
                     /note="derived from pBBR1MCS-2"
     CDS             complement(4127..4921)
                     /codon_start=1
                     /gene="nptII"
                     /product="NptII"
                     /label=nptII
                     /note="kanamycin resistance determinant"
                     /protein_id="ANH21054.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            complement(4127..4921)
                     /gene="nptII"
                     /label=nptII