LOCUS       Exported                6236 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pYAA-ZP-MCS, complete sequence.
ACCESSION   EU882163
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6236)
  AUTHORS   Zaltsman AA.
  TITLE     pYAA-ZP-MCS: plasmid for expression, localization, and tagging in 
            yeast with an adenine biosynthesis reporter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6236)
  AUTHORS   Zaltsman AA.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2008) Biochemistry and Cell Biology, State 
            University of New York, Stony Brook, NY 11794, USA
REFERENCE   3  (bases 1 to 6236)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6236
                     /organism="Expression vector pYAA-ZP-MCS"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
                     /label=PCR_primers=fwd_name: M13F, rev_name: M13R
                     /note="PCR_primers=fwd_name: M13F, rev_name: M13R"
                     /db_xref="taxon:552403"
     promoter        199..574
                     /gene="S. cerevisiae ADE2"
                     /label=ADE2 promoter
     CDS             575..2290
                     /codon_start=1
                     /gene="ADE"
                     /allele="1"
                     /product="ADE-1"
                     /label=ADE
                     /note="involved in adenine biosynthesis; 
                     phosphoribosylamino-imidazole-carboxylase"
                     /protein_id="ACG61339.1"
                     /translation="MDSRTVGILGGGQLGRMIVEAANRLNIKTVILDAENSPAKQISNS
                     NDHVNGSFSNPLDIEKLAEKCDVLTIEIEHVDVPTLKNLQVKHPKLKIYPSPETIRLIQ
                     DKYIQKEHLIKNGIAVTQSVPVEQASETSLLNVGRDLGFPFVLKSRTLAYDGRGNFVVK
                     NKEMIPEALEVLKDRPLYAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLC
                     YAPARVPDSVQLKAKLLAENAIKSFPGCGIFGVEMFYLETGELLINEIAPRPHNSGHYT
                     IDACVTSQFEAHLRSILDLPMPKNFTSFSTITTNAIMLNVLGDKHTKDKELETCERALA
                     TPGSSVYLYGKESRPNRKVGHINIIASSMAECEQRLNYITGRTDIPIKISVAQKLDLEA
                     MVKPLVGIIMGSDSDLPVMSAACAVLKDFGVPFEVTIVSAHRTPHRMSAYAISASKRGI
                     KTIIAGAGGAAHLPGMVAAMTPLPVIGVPVKGSCLDGVDSLHSIVQMPRGVPVATVAIN
                     NSTNAALLAVRLLGAYDSSYTTKMEQFLLKQEEEVLVKAQKLETVGYEAYLENK"
     gene            575..2290
                     /gene="ADE"
                     /allele="1"
                     /label=ADE
     rep_origin      complement(2495..2950)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     3095..3111
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3121..3139
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3241..3396
                     /note="multiple cloning site containing ISCEI, PIPSPI, 
                     ICEUI, and IPPOI unique sites"
     primer_bind     complement(3410..3426)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(3463..3481)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3502..3518)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3526..3542
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3550..3580)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3595..3616
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      3831..4285
                     /label=ColE1
                     /note="ColE1"
     rep_origin      complement(3904..4492)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4663..5523)
                     /codon_start=1
                     /gene="BLA"
                     /product="BLA"
                     /label=BLA
                     /note="AMP-R"
                     /protein_id="ACG61340.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(4663..5523)
                     /gene="BLA"
                     /label=BLA
     promoter        complement(5524..5628)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5636..6236
                     /label=CEN6 for replication in Saccharomyces cerevisiae
                     /note="CEN6 for replication in Saccharomyces cerevisiae"
     misc_feature    5664..6167
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"