LOCUS       Exported                7014 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pXZ240, complete sequence.
ACCESSION   AF316555
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Zeng X, Herndon AM, Hu JC.
  TITLE     Buried asparagines determine the dimerization specificities of 
            leucine zipper mutants
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 94 (8), 3673-3678 (1997)
  PUBMED    9108036
REFERENCE   2  (bases 1 to 7014)
  AUTHORS   Marino-Ramirez L, Hu JC.
  TITLE     Using lambda repressor fusions to isolate and characterize 
            self-assembling domains
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7014)
  AUTHORS   Marino-Ramirez L, Hu JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2000) Biochemistry & Biophysics, Texas A&M 
            University, TAMU 2128, College Station, TX 77843-2128, USA
REFERENCE   4  (bases 1 to 7014)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="Cloning vector pXZ240"
                     /mol_type="other DNA"
                     /db_xref="taxon:151161"
     regulatory      5..48
                     /regulatory_class="promoter"
                     /note="HH7107; weak constitutive promoter derived from 
                     lacUV5"
                     /citation="[1]"
     CDS             73..501
                     /codon_start=1
                     /product="cI binding domain"
                     /label=cI binding domain
                     /note="cI-DBD"
                     /protein_id="AAK07890.1"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTDRSRRFTTS"
     terminator      2543..2590
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             4075..4266
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    4368..4508
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4694..5282)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      5393..5906
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(5948..6808)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="bla"
                     /protein_id="AAK07891.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6809..6913)
                     /gene="bla"
                     /label=AmpR promoter