LOCUS       Exported                6416 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pXtale, complete sequence.
ACCESSION   KY511412
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Pesce C, Koebnik R.
  TITLE     TALE expression vector for clade-1 xanthomonads
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6416)
  AUTHORS   Pesce C, Koebnik R.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2017) UMR 186 IPME, IRD Montpellier, 911, Avenue 
            Agropolis, Montpellier, Languedoc-Roussillon 34394, France
REFERENCE   3  (bases 1 to 6416)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Cloning vector pXtale"
                     /lab_host="Xanthomonas translucens"
                     /mol_type="other DNA"
                     /db_xref="taxon:2021820"
     rep_origin      1023..1792
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1793..2455
                     /codon_start=1
                     /product="REP"
                     /label=REP
                     /note="required for plasmid replication"
                     /protein_id="ASM90836.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     3162..3178
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3188..3206
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            complement(3245..5100)
                     /gene="TAL"
                     /label=TAL
     CDS             complement(3245..4912)
                     /codon_start=1
                     /gene="TAL"
                     /product="TAL effector repeat array acceptor"
                     /label=TAL
                     /note="TAL effector derivative without repeat array"
                     /protein_id="ASM90837.1"
                     /translation="MEPIRSRTPSTARELQAGSQPDAVQPIADRLVSTPASSPLDGLPA
                     RRMVSRTSPPPSPARSPAFSAGSLSGLLRQQIDPSLFAGSPFDSLPSFGAARAESAPGE
                     GDEVQSALRAVDDPQPSASAAITAAPPRTKAAARRRSAQTSDALPAAHVDLGTFGYSQQ
                     QQEKIKPKVRSTVAQHHEALVGHGFTHAHIVELSKHPPALGTIAARYSEMIAALPEATH
                     EDIVGVGKQKSGARALEALLTVAEELRAPPLQLVTGQLLKIAKRGGVNAVEVRPASSST
                     RVSPDPALAALTNDRLVALACLGGRPALDAVKKGLPHAPALVTRTHNRVPEGTAHLVAD
                     HAQVVRVLGFFQCHSHPAQAFHEAMTRFEMSREGLLQLFRRVGVTELEAISGTLPPASQ
                     RWHRILQALGVKGAKPSSASAQTPSQESVDAFADSLERELDAPSPIHEADRARASNRKR
                     SRSESSVNRSSAQQAAEVFVPEQRDAPPLLPLSSWGVKRQRTRIGGLPDPGMPTDGELA
                     ASSAAFLEQDADPFAGAAEDFPVFDQEEIAWLMTLLPH"
     primer_bind     complement(4937..4953)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4983..5001)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5022..5038)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5046..5062
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      complement(5070..5100)
                     /regulatory_class="promoter"
                     /gene="TAL"
     promoter        complement(5070..5100)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5115..5136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5406..5434
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             5623..6156
                     /codon_start=1
                     /product="gentamicin resistance protein"
                     /label=gentamicin resistance protein
                     /note="gentamicin-3-acetyltransferase"
                     /protein_id="ASM90838.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"