LOCUS       Exported                7947 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pXsp8, complete sequence.
ACCESSION   KC817027
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7947)
  AUTHORS   Kurosawa K, Wewetzer SJ, Sinskey AJ.
  TITLE     Engineering xylose metabolism in triacylglycerol-producing 
            Rhodococcus opacus for lignocellulosic fuel production
  JOURNAL   Biotechnol Biofuels 6 (1), 134 (2013)
  PUBMED    24041310
REFERENCE   2  (bases 1 to 7947)
  AUTHORS   Kurosawa K.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-2013) Biology, Massachusetts Institute of 
            Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 7947)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7947
                     /organism="Shuttle vector pXsp8"
                     /mol_type="other DNA"
                     /db_xref="taxon:1403749"
     primer_bind     341..357
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        364..382
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(705..1871)
                     /codon_start=1
                     /gene="xylA"
                     /product="xylose isomerase"
                     /label=xylA
                     /protein_id="AGW51574.1"
                     /translation="MSFQPTPEDRFTFGLWTVGWQGRDPFGDATRPALDPVETVQRLAE
                     LGAYGVTFHDDDLIPFGSSDTERESHIKRFRQALDATGMTVPMATTNLFTHPVFKDGGF
                     TANDRDVRRYALRKTIRNIDLAAELGAKTYVAWGGREGAESGGAKDVRDALDRMKEAFD
                     LLGEYVIAQGYDLRFAIEPKPNEPRGDILLPTVGHALAFIERLERPELYGVNPEVGHEQ
                     MAGLNFPHGIAQALWAGKLFHIDLNGQSGIKYDQDLRFGAGDLRAAFWLVDLLETAGYE
                     GPRHFDFKPPRTEDFDGVWASAAGCMRNYLILKDRAAAFRADPEVQEALRAARLDQLAQ
                     ATAADGLDALLADRSAFEDFDVDAAAARGMAFEHLDQLAMDHLLGARG"
     gene            complement(705..1871)
                     /gene="xylA"
                     /label=xylA
     CDS             2068..4005
                     /codon_start=1
                     /gene="xylB"
                     /product="xylulose kinase"
                     /label=xylB
                     /protein_id="AGW51575.1"
                     /translation="MSAAEGPLVVGVDTSTQSTKALVVDAATGQVVASGQAPHTVTTGG
                     GRESDPRQWWDALCEALRQCGDAAHEAAAISVGGQQHGLVTLDERGEPVRPALLWNDVR
                     SAPQARRLVEELGGPKAWAERTGSVPGASFTASKWAWLAEHEPEAARATRAVRLPHDYL
                     TERLTGQAVTDRGDASGTGWWASGTERYDEDTLAHLGLDPALLPRVVRPGEVAGTVRDG
                     HGLPFSKGTLVASGTGDNAAAALGLGLLPGTPVLSLGTSGTVYAVSRHRPADPTGTVAG
                     FADARGDWLPLACTLNCTLAVDRVATLLGLDREAVAPGGSVTLLPFLDGERTPDLPNAS
                     GLLHGLRHDTTPGQLLQAAYDGAVHSLLGALDLVLDEDADRDSPLLLIGGGARGHAWQQ
                     TVRRLSGRPVQILRLAEEEAKELREEARRAAEQHRELAESAAQQVRNDAESYATERKAK
                     AEDEGSRIVEKAKGEASQLRADAQKDAQSKREEADALFEETRAKAAQAAADFETNLAKR
                     REQSERDLASRQQKAEKRLAEIEHRAEQLRLEAEKLRTDAERRARQTVETAQRQAEDIV
                     ADANAKADRIRSESERELAALTNRRDSINAQLTNVREMLATLTGAAVAAAGTTGEDEPV
                     SRGVPAQQTR"
     gene            2068..4005
                     /gene="xylB"
                     /label=xylB
     primer_bind     complement(4111..4127)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4135..4151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4159..4189)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4204..4225
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4711..4739
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     oriT            5530..5639
                     /note="incP origin of transfer"
     CDS             6337..7788
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein"
                     /label=repA
                     /protein_id="AGW51576.1"
                     /translation="MSLQTHAQNAHTKTPTRVERDRGGLDNTPASASDRDLLRDHLGRD
                     VLHGSVTRDFKKAYRRNADGTNSPRMYRFETDALGRCEYAMLTTKQYAAVLVVDVDQVG
                     TAGGDPADLNPYVRDVVRSLITHSVGPAWVGINPTNGKAQFIWLIDPVYADRNGKSAQM
                     KLLAATTRVLGELLDHDPHFSHRFSRNPFYTGKAPTAYRWYRQHNRVMRLGDLIKQVRD
                     MAGHDQFNPTPRQQFSSGRELINAVKTRREEAQAFKALAQDVDAEIAGGLDQYDPELID
                     GVRVLWIVQGTAARDETAFRHALKTGHRLRQQGQRLTDAAIIDAYEHAYNVAHTHGGAG
                     RDNEMPPMRDRQTMARRVRGYVAQSKSETYSGSNAPGKATSSERKALATMGRRGGQKAA
                     QRWKTDPEGKYAQAQRSKLEKTHRKKKAQGRSTKSRISQMVNDQYFQTGTVPTWAEIGA
                     EVGVSRATVARHVAELKKSGDYPDV"
     gene            6337..7788
                     /gene="repA"
                     /label=repA