LOCUS       Exported                5267 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Plant binary vector pXS2pat-Strep, complete sequence.
ACCESSION   HM439362
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Cao FQ, Werner AK, Dahncke K, Romeis T, Liu LH, Witte CP.
  TITLE     Identification and characterization of proteins involved in rice 
            urea and arginine catabolism
  JOURNAL   Plant Physiol. 154 (1), 98-108 (2010)
  PUBMED    20631318
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Cao FQ, Dahncke K, Liu LH, Romeis T, Werner AK, Witte C-P.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2010) Dahlem Centre of Plant Science, Department 
            of Plant Biochemistry, Freie Universitaet Berlin, 
            Koenigin-Luise-Str. 12-16, Berlin 14195, Germany
REFERENCE   3  (bases 1 to 5267)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Plant binary vector pXS2pat-Strep"
                     /mol_type="other DNA"
                     /db_xref="taxon:881642"
     primer_bind     complement(45..61)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      57..273
                     /regulatory_class="terminator"
                     /note="p35S"
     misc_feature    344..369
                     /label=T-DNA right border
                     /note="T-DNA right border"
     misc_feature    344..368
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      480..1189
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      484..1192
                     /label=RK2
                     /note="RK2"
     promoter        1263..1354
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1355..2215
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="ADM88589.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2284..2976
                     /label=ColE1
                     /note="ColE1"
     rep_origin      2386..2974
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3285..3310
                     /label=T-DNA left border
                     /note="T-DNA left border"
     misc_feature    3286..3310
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      complement(3385..3669)
                     /regulatory_class="terminator"
                     /note="pnos"
     terminator      3404..3656
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3676..4227)
                     /codon_start=1
                     /product="phosphinothricin N-acetyltransferase"
                     /label=phosphinothricin N-acetyltransferase
                     /protein_id="ADM88590.1"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     regulatory      complement(4249..4559)
                     /regulatory_class="promoter"
                     /note="pnos"
     promoter        complement(4270..4449)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     regulatory      4569..5267
                     /regulatory_class="promoter"
                     /note="p35SS"
     promoter        5219..5265
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"