LOCUS       Exported                6000 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Plant binary vector pXNS2pat-YFP, complete sequence.
ACCESSION   KF499077
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Dahncke K, Witte CP.
  TITLE     Plant purine nucleoside catabolism employs a guanosine deaminase 
            required for the generation of xanthosine in Arabidopsis
  JOURNAL   Plant Cell 25 (10), 4101-4109 (2013)
  PUBMED    24130159
REFERENCE   2  (bases 1 to 6000)
  AUTHORS   Dahncke K, Witte C-P.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2013) Department of Plant Biochemistry, Freie 
            Universitaet Berlin, Dahlem Centre of Plant Sciences, 
            Koenigin-Luise-Str. 12-16, Berlin 14195, Germany
REFERENCE   3  (bases 1 to 6000)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Plant binary vector pXNS2pat-YFP"
                     /mol_type="other DNA"
                     /db_xref="taxon:1411100"
     CDS             30..746
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             30..745
                     /codon_start=1
                     /gene="eYFP"
                     /product="eYFP"
                     /label=eYFP
                     /note="creates an N-terminal YFP-fusion protein"
                     /protein_id="AGY49277.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     gene            30..745
                     /gene="eYFP"
                     /label=eYFP
     primer_bind     complement(778..794)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      789..1006
                     /regulatory_class="terminator"
                     /note="p35S"
     misc_feature    1077..1102
                     /label=T-DNA right border
                     /note="T-DNA right border"
     misc_feature    1077..1101
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      1213..1922
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      1217..1925
                     /label=RK2
                     /note="RK2"
     promoter        1996..2087
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2088..2948
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="AGY49278.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3017..3709
                     /label=ColE1
                     /note="ColE1"
     rep_origin      3119..3707
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4018..4043
                     /label=T-DNA left border
                     /note="T-DNA left border"
     misc_feature    4019..4043
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      complement(4118..4402)
                     /regulatory_class="terminator"
                     /note="pnos"
     terminator      4137..4389
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4409..4960)
                     /codon_start=1
                     /product="phosphinotricin N-acetyltransferase"
                     /label=phosphinotricin N-acetyltransferase
                     /protein_id="AGY49279.1"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     regulatory      complement(4982..5292)
                     /regulatory_class="promoter"
                     /note="pnos"
     promoter        complement(5003..5182)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     regulatory      5302..6000
                     /regulatory_class="promoter"
                     /note="p35SS"
     promoter        5952..5998
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"