LOCUS       Exported                5316 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Plant binary vector pXNS1pat-Strep, complete sequence.
ACCESSION   HM439359
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Cao FQ, Werner AK, Dahncke K, Romeis T, Liu LH, Witte CP.
  TITLE     Identification and characterization of proteins involved in rice 
            urea and arginine catabolism
  JOURNAL   Plant Physiol. 154 (1), 98-108 (2010)
  PUBMED    20631318
REFERENCE   2  (bases 1 to 5316)
  AUTHORS   Cao FQ, Dahncke K, Liu LH, Romeis T, Werner AK, Witte C-P.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2010) Dahlem Centre of Plant Science, Department 
            of Plant Biochemistry, Freie Universitaet Berlin, 
            Koenigin-Luise-Str. 12-16, Berlin 14195, Germany
REFERENCE   3  (bases 1 to 5316)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5316
                     /organism="Plant binary vector pXNS1pat-Strep"
                     /mol_type="other DNA"
                     /db_xref="taxon:881639"
     misc_feature    39..62
                     /label=StrepII epitope
                     /note="StrepII epitope"
     CDS             39..62
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(94..110)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      105..322
                     /regulatory_class="terminator"
                     /note="p35S"
     misc_feature    393..418
                     /label=T-DNA right border
                     /note="T-DNA right border"
     misc_feature    393..417
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      529..1238
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      533..1241
                     /label=RK2
                     /note="RK2"
     promoter        1312..1403
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1404..2264
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="ADM88583.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2333..3025
                     /label=ColE1
                     /note="ColE1"
     rep_origin      2435..3023
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3334..3359
                     /label=T-DNA left border
                     /note="T-DNA left border"
     misc_feature    3335..3359
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      complement(3434..3718)
                     /regulatory_class="terminator"
                     /note="pnos"
     terminator      3453..3705
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3725..4276)
                     /codon_start=1
                     /product="phosphinothricin N-acetyltransferase"
                     /label=phosphinothricin N-acetyltransferase
                     /protein_id="ADM88584.1"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     regulatory      complement(4298..4608)
                     /regulatory_class="promoter"
                     /note="pnos"
     promoter        complement(4319..4498)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     regulatory      4618..5316
                     /regulatory_class="promoter"
                     /note="p35SS"
     promoter        5268..5314
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"