LOCUS       Exported                5073 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Dicistronic cloning vector pXLJ Con, complete sequence.
ACCESSION   EU919738
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Mokrejs M, Vopalensky V, Kolenaty O, Masek T, Feketova Z, Sekyrova 
            P, Skaloudova B, Kriz V, Pospisek M.
  TITLE     IRESite: the database of experimentally verified IRES structures 
            (www.iresite.org)
  JOURNAL   Nucleic Acids Res. 34 (DATABASE ISSUE), D125-D130 (2006)
  PUBMED    16381829
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Masek T, Mokrejs M, Vopalensky V, Pospisek M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUL-2008) Genetics and Microbiology, Faculty of 
            Science, Charles University in Prague, Vinicna 5, Prague 128 44, 
            Czech Republic
REFERENCE   3  (bases 1 to 5073)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Dicistronic cloning vector pXLJ Con"
                     /mol_type="other DNA"
                     /db_xref="taxon:556550"
     misc_feature    join(351..2049,4585..4829)
                     /note="sequence derived from plasmid originally published 
                     in Borman and Jackson 1992"
     promoter        807..911
                     /gene="bla"
                     /label=AmpR promoter
     CDS             912..1772
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /EC_number="3.5.2.6"
                     /label=bla
                     /note="beta-lactam hydrolase"
                     /protein_id="ACH71402.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            912..1772
                     /gene="bla"
                     /label=bla
     rep_origin      1867..2549
                     /label=ColE1
                     /note="ColE1"
     rep_origin      1943..2531
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2783..2801
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            2838..4186
                     /gene="B2"
                     /label=B2
     CDS             2838..4016
                     /codon_start=1
                     /gene="B2"
                     /product="B2 cyclin"
                     /label=B2
                     /note="sequence derived from Xenopus laevis"
                     /protein_id="ACH71403.1"
                     /translation="MATRRAAIPREADNILGGAMRSKVQMNSRRAALGEIGNKVTVRGK
                     PPAVKQSSNAVAKPSKMAATKVANVKTKHVPVKPVVAEAAPKVPSPVPMDVSLKEEELC
                     QAFSDALTSVEDIDADDGGNPQLCSDYVMDIYNYLKQLEVQQSVHPCYLEGKEINERMR
                     AILVDWLVQVHSRFQLLQETLYMGVAIMDRFLQVQPVSRSKLQLVGVTSLLIASKYEEM
                     YTPEVADFVYITDNAYTASQIREMEMIILRLLNFDLGRPLPLHFLRRASKSCSADAEQH
                     TLAKYLMELTLIDYEMVHIKPSEIAAAALCLSQKILGQGTWGTTQHYYTGYTEGDLQLI
                     MKHMAKNITKVNQNLTKHVAVRNKYASSKLMKISTLPQLMAPLITELAASLS"
     3UTR           4017..4186
                     /gene="B2"
     gene            4206..5057
                     /gene="NS1"
                     /label=NS1
     CDS             4206..4886
                     /codon_start=1
                     /gene="NS1"
                     /product="NS"
                     /label=NS1
                     /note="structural protein; N-terminally truncated influenza
                     NS1 protein; first 3 amino acids originate from plasmid 
                     polylinker"
                     /protein_id="ACH71404.1"
                     /translation="MDPSFQVDCFLWHVRKRVADQELGDAPFLDRLRRDQKSLRGRGST
                     LGLDIETATRAGKQIVERILKEESDEALKMTMASVPASRYLTDMTLEEMSRDWSMLIPK
                     QKVAGPLCIRMDQAIMDKNIILKANFSVIFDRLETLILLRAFTEEGAIVGEISPLPSLP
                     GHTAEDVKNAVGVLIGGLEWNDNTVRVSETLQRFAWRSSNENGRPPLTPKQKREMAGTI
                     RSEV"
     3UTR           4887..5057
                     /gene="NS1"
                     /note="sequence derived from Influenza virus genomic 
                     segment 8"
     promoter        complement(5072..17)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"