LOCUS       Exported                5611 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Dicistronic cloning vector pXL-Id, complete sequence.
ACCESSION   EU919739
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Mokrejs M, Vopalensky V, Kolenaty O, Masek T, Feketova Z, Sekyrova 
            P, Skaloudova B, Kriz V, Pospisek M.
  TITLE     IRESite: the database of experimentally verified IRES structures 
            (www.iresite.org)
  JOURNAL   Nucleic Acids Res. 34 (DATABASE ISSUE), D125-D130 (2006)
  PUBMED    16381829
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   Masek T, Mokrejs M, Vopalensky V, Pospisek M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUL-2008) Genetics and Microbiology, Faculty of 
            Science, Charles University in Prague, Vinicna 5, Prague 128 44, 
            Czech Republic
REFERENCE   3  (bases 1 to 5611)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Dicistronic cloning vector pXL-Id"
                     /mol_type="other DNA"
                     /db_xref="taxon:556549"
     misc_feature    join(1..3799,5173..5611)
                     /note="sequence derived from plasmid originally published 
                     in Meignin et. al. 2003"
     promoter        807..911
                     /gene="bla"
                     /label=AmpR promoter
     CDS             912..1772
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /EC_number="3.5.2.6"
                     /label=bla
                     /note="beta-lactam hydrolase"
                     /protein_id="ACH71405.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            912..1772
                     /gene="bla"
                     /label=bla
     rep_origin      1867..2549
                     /label=ColE1
                     /note="ColE1"
     rep_origin      1943..2531
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2783..2801
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            2838..4186
                     /gene="B2"
                     /label=B2
     CDS             2838..4016
                     /codon_start=1
                     /gene="B2"
                     /product="B2 cyclin"
                     /label=B2
                     /note="cyclin"
                     /protein_id="ACH71406.1"
                     /translation="MATRRAAIPREADNILGGAMRSKVQMNSRRAALGEIGNKVTVRGK
                     PPAVKQSSNAVAKPSKMAATKVANVKTKHVPVKPVVAEAAPKVPSPVPMDVSLKEEELC
                     QAFSDALTSVEDIDADDGGNPQLCSDYVMDIYNYLKQLEVQQSVHPCYLEGKEINERMR
                     AILVDWLVQVHSRFQLLQETLYMGVAIMDRFLQVQPVSRSKLQLVGVTSLLIASKYEEM
                     YTPEVADFVYITDNAYTASQIREMEMIILRLLNFDLGRPLPLHFLRRASKSCSADAEQH
                     TLAKYLMELTLIDYEMVHIKPSEIAAAALCLSQKILGQGTWGTTQHYYTGYTEGDLQLI
                     MKHMAKNITKVNQNLTKHVAVRNKYASSKLMKISTLPQLMAPLITELAASLS"
     3UTR           4017..4186
                     /gene="B2"
     misc_feature    4218..4741
                     /label=Idefix internal ribosome entry site
                     /note="Idefix internal ribosome entry site"
     gene            4726..5595
                     /gene="NS1"
                     /label=NS1
     CDS             4726..5424
                     /codon_start=1
                     /gene="NS1"
                     /product="NS"
                     /label=NS1
                     /note="structural protein; N-terminally truncated influenza
                     NS1 protein; first 5 amino acids originate from Idefix 
                     retrotransposon and next 4 amino acids originate from 
                     plasmid polylinker"
                     /protein_id="ACH71407.1"
                     /translation="MAVPQRIDPSFQVDCFLWHVRKRVADQELGDAPFLDRLRRDQKSL
                     RGRGSTLGLDIETATRAGKQIVERILKEESDEALKMTMASVPASRYLTDMTLEEMSRDW
                     SMLIPKQKVAGPLCIRMDQAIMDKNIILKANFSVIFDRLETLILLRAFTEEGAIVGEIS
                     PLPSLPGHTAEDVKNAVGVLIGGLEWNDNTVRVSETLQRFAWRSSNENGRPPLTPKQKR
                     EMAGTIRSEV"
     3UTR           5425..5595
                     /gene="NS1"
                     /note="sequence derived from Influenza virus genomic 
                     segment 8"
     promoter        complement(5610..17)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"