LOCUS       Exported                4425 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pXICL, complete sequence.
ACCESSION   GU046314
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Ness JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2009) DNA2.0, Inc., 1430 OBrien Drive, Suite E, 
            Menlo Park, CA 94025, USA
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Expression vector pXICL"
                     /lab_host="Candida tropicalis"
                     /mol_type="other DNA"
                     /db_xref="taxon:684758"
     regulatory      13..1490
                     /regulatory_class="promoter"
                     /note="ICL promoter"
     CDS             1493..2203
                     /codon_start=1
                     /product="mCherry"
                     /label=mCherry
                     /protein_id="ACY24904.1"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     regulatory      2212..2508
                     /regulatory_class="terminator"
                     /note="ICL terminator"
     promoter        2510..2921
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     regulatory      2515..2926
                     /regulatory_class="promoter"
                     /note="TEF1 promoter"
     regulatory      2927..2994
                     /regulatory_class="promoter"
                     /note="EM7 promoter"
     promoter        2929..2976
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2995..3369
                     /codon_start=1
                     /product="zeocin resistance protein"
                     /label=zeocin resistance protein
                     /protein_id="ACY24905.1"
                     /translation="MSKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      3370..3599
                     /regulatory_class="terminator"
                     /note="CYC1 terminator"
     terminator      3435..3682
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(3698..4371)
                     /direction=LEFT
                     /label=pUC origin
                     /note="pUC origin"
     rep_origin      complement(3757..4345)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"