LOCUS       Exported                6664 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Promoter-probe vector pXH204, complete sequence.
ACCESSION   AY652438
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6664)
  AUTHORS   He X, Bhateja M, Fuqua C.
  TITLE     Promoter-probe cassettes with the gusA (beta-glucuronidase) reporter
            gene and several different antibiotic resistance markers
  JOURNAL   J. Microbiol. Methods 60 (2), 281-283 (2005)
  PUBMED    15590103
REFERENCE   2  (bases 1 to 6664)
  AUTHORS   He X, Bhateja M, Fuqua C.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2004) Biology, Indiana University, 1001 E. 3rd 
            St., Jordan Hall, Bloomington, IN 47405, USA
REFERENCE   3  (bases 1 to 6664)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6664
                     /organism="Promoter-probe vector pXH204"
                     /mol_type="other DNA"
                     /db_xref="taxon:284491"
     misc_feature    1..51
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             76..1914
                     /codon_start=1
                     /gene="GusA"
                     /product="beta-glucuronidase"
                     /label=GusA
                     /note="reporter; hydrolyzes beta-glucuronic acid and 
                     analogs"
                     /protein_id="AAT72059.1"
                     /translation="MESLAAGQSLMLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWW
                     ESALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHY
                     GKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDEN
                     GKKKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDV
                     SVELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGI
                     RSVAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSH
                     YPYAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHL
                     QAIKELIARDKNHPSVVMWSIANEPDTRPQVHGNISPLAEATRKLDPTRPITCVNVMFC
                     DAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNK
                     KGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            76..1914
                     /gene="GusA"
                     /label=GusA
     CDS             2766..3557
                     /codon_start=1
                     /gene="SmSp"
                     /product="streptomycin/spectinomycin adenyltransferase"
                     /label=SmSp
                     /note="imparts streptomycin and spectinomycin resistance in
                     compatible hosts"
                     /protein_id="AAT72060.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            2766..3557
                     /gene="SmSp"
                     /label=SmSp
     misc_feature    3928..3978
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(3984..4002)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4021..4037)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4045..4061
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4069..4099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4114..4135
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4423..5011)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5182..6042)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="Bla; imparts resistance to beta-lactam antibiotics 
                     in compatible hosts"
                     /protein_id="AAT72061.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(5182..6042)
                     /gene="bla"
                     /label=bla
     promoter        complement(6043..6147)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6621..6637
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6640..6658
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"