LOCUS       Exported                3239 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pXEN, complete sequence.
ACCESSION   EU267939
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Czirjak G, Vuity D, Enyedi P.
  TITLE     Phosphorylation-dependent binding of 14-3-3 proteins controls TRESK 
            regulation
  JOURNAL   J. Biol. Chem. 283 (23), 15672-15680 (2008)
  PUBMED    18397886
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Czirjak G, Vuity D, Enyedi P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2007) Department of Physiology, Semmelweis 
            University, Puskin str. 9, Budapest 1088, Hungary
REFERENCE   3  (bases 1 to 3239)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Cloning vector pXEN"
                     /mol_type="other DNA"
                     /note="pBluescript II SK- derivative; constructed from pEXO
                     by introducing multiple cloning site; contains Xenopus 
                     globin 5 and 3 UTRs"
                     /db_xref="taxon:485428"
     promoter        9..27
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    9..26
                     /label=SP6 primer
                     /note="SP6 primer"
     5UTR           27..60
                     /label=derived from Xenopus globin
                     /note="derived from Xenopus globin"
     misc_feature    61..114
                     /label=multiple cloning site
                     /note="multiple cloning site"
     3UTR           115..300
                     /label=derived from Xenopus globin
                     /note="derived from Xenopus globin"
     3UTR           132..257
                     /label=Xenopus globin 3-UTR
                     /note="3-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     misc_feature    327..3239
                     /label=pBluescript II SK- plasmid backbone
                     /note="pBluescript II SK- plasmid backbone"
     primer_bind     complement(341..357)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(394..412)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(433..449)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    457..473
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(481..511)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    526..547
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      653..1533
                     /label=ColE1
                     /note="ColE1"
     rep_origin      complement(835..1423)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1594..2454)
                     /codon_start=1
                     /product="AmpR"
                     /label=AmpR
                     /note="confers ampicillin resistance"
                     /protein_id="ABX71214.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2455..2559)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    2585..3041
                     /label=f1 origin
                     /note="f1 origin"
     rep_origin      2586..3041
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     3182..3198
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3208..3226
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"