LOCUS       Exported                5332 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Plant binary vector pXCS-HAStrep, complete sequence.
ACCESSION   AY457636
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Witte CP, Noel LD, Gielbert J, Parker JE, Romeis T.
  TITLE     Rapid one-step protein purification from plant material using the 
            eight-amino acid StrepII epitope
  JOURNAL   Plant Mol. Biol. 55 (1), 135-147 (2004)
  PUBMED    15604670
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Witte C-P., Noel L, Parker JE, Romeis T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2003) Plant-Microbe Interactions, 
            Max-Planck-Institute for Plant Breeding Research, Carl-von-Linne-Weg
            10, Koeln D-50829, Germany
REFERENCE   3  (bases 1 to 5332)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Plant binary vector pXCS-HAStrep"
                     /mol_type="other DNA"
                     /db_xref="taxon:262647"
     misc_feature    22..48
                     /label=encodes HA epitope
                     /note="encodes HA epitope"
     CDS             22..48
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    61..87
                     /label=encodes StrepII epitope
                     /note="encodes StrepII epitope"
     CDS             61..84
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     regulatory      90..306
                     /regulatory_class="terminator"
                     /note="pA35S"
     misc_feature    complement(377..402)
                     /label=T-DNA right border
                     /note="T-DNA right border"
     misc_feature    377..401
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      513..1222
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      complement(517..1225)
                     /direction=LEFT
                     /label=RK2
                     /note="RK2"
     promoter        1296..1387
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1388..2248
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AAS13538.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            1388..2248
                     /gene="bla"
                     /label=bla
     rep_origin      2317..3009
                     /label=ColE1
                     /note="ColE1"
     rep_origin      2419..3007
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3318..3343
                     /label=T-DNA left border
                     /note="T-DNA left border"
     misc_feature    3319..3343
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      complement(3418..3702)
                     /regulatory_class="terminator"
                     /note="pnos"
     terminator      3437..3689
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3709..4260)
                     /codon_start=1
                     /gene="pat"
                     /product="pat"
                     /label=pat
                     /note="confers resistance to Basta/PPT"
                     /protein_id="AAS13537.1"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     gene            complement(3709..4260)
                     /gene="pat"
                     /label=pat
     regulatory      complement(4282..4592)
                     /regulatory_class="promoter"
                     /note="pnos"
     promoter        complement(4303..4482)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     regulatory      4602..5300
                     /regulatory_class="promoter"
                     /note="35SS"
     promoter        5252..5298
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     5302..5318
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"