LOCUS       Exported                3551 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pXcmkn12 DNA, complete sequence.
ACCESSION   AB107950
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Yasuda S.
  TITLE     Cloning Vector pXcmkn12
  JOURNAL   Published Only in Database (2003)
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Yasuda S, Tamura S, Yamamoto M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2003) Seiichi Yasuda, National Instutute of 
            Genetics, Microbial Genetics; Yata, 1111, Mishima, Shizuoka 
            411-8540, Japan (E-mail:cvector@lab.nig.ac.jp, Tel:81-55-981-6759, 
            Fax:81-55-981-6763)
REFERENCE   3  (bases 1 to 3551)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Cloning vector pXcmkn12"
                     /mol_type="other DNA"
                     /note="This vector was constructed by S. Chandrasegaran, 
                     and is an improved version of pCBC1 (Cha, J., Bishai W. and
                     Chandrasegaran, s. Gene 136 (1993) 369-370)"
                     /db_xref="taxon:227918"
     rep_origin      complement(183..771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(942..1802)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="BAC75545.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(942..1802)
                     /gene="bla"
                     /label=bla
     promoter        complement(1803..1907)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2381..2397
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2401..2430
                     /label=multi cloning site-1
                     /note="multi cloning site-1"
     misc_feature    2435..2449
                     /label=XcmI site-1
                     /note="XcmI site-1"
     misc_feature    2463..3278
                     /label=Kanamycin resistance gene
                     /note="Kanamycin resistance gene"
     CDS             2463..3278
                     /codon_start=1
                     /gene="aph(3)-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    3282..3296
                     /label=XcmI site-2
                     /note="XcmI site-2"
     misc_feature    3295..3322
                     /label=multi cloning site-2
                     /note="multi cloning site-2"
     primer_bind     complement(3332..3348)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3356..3372
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3380..3410)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3425..3446
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."