LOCUS       Exported                5008 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pX1918G, complete sequence.
ACCESSION   U17501
VERSION     .
KEYWORDS    bata-lactamase (bla); catechol-2,3-dioxygenase (xylE); gentamy...
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-1994) Herbert P. Schweizer, Microbiology & 
            Infectious Diseases, University of Calgary, 3330 Hospital Drive 
            N.W., Calgary, Alberta T2N 4N1, Canada
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Schweizer HP, Hoang TT.
  TITLE     An improved system for gene replacement and xylE fusion analysis in 
            Pseudomonas aeruginosa
  JOURNAL   Gene 158 (1), 15-22 (1995)
  PUBMED    7789804
REFERENCE   3  (bases 1 to 5008)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Cloning vector pX1918G"
                     /plasmid="pX1918G"
                     /host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:37981"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    399..455
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     misc_feature    399..454
                     /label=polylinker
                     /note="polylinker"
     CDS             665..1588
                     /codon_start=1
                     /gene="xylE"
                     /product="catechol-2,3-dioxygenase"
                     /label=xylE
                     /protein_id="AAA57327.1"
                     /translation="MNKGVMRPGHVQLRVLDMSKALEHYVELLGLIEMDRDDQGRVYLK
                     AWTEVDKFSLVLREADEPGMDFMGFKVVDEDALRQLERDLMAYGCAVEQLPAGELNSCG
                     RRVRFQAPSGHHFELYADKEYTGKWGLNDVNPEAWPRDLKGMAAVRFDHALMYGDELPA
                     TYDLFTKVLGFYLAEQVLDENGTRVAQFLSLSTKAHDVAFIHHPEKGRLHHVSFHLETW
                     EDLLRAADLISMTDTSIDIGPTRHGLTHGKTIYFFDPSGNRNEVFCGGDYNYPDHKPVT
                     WTTDQLGKAIFYHDRILNERFMTVLT"
     gene            665..1588
                     /gene="xylE"
                     /label=xylE
     promoter        1704..1732
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             1921..2454
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase-3-1"
                     /label=aacC1
                     /protein_id="AAA57328.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     gene            1921..2454
                     /gene="aacC1"
                     /label=aacC1
     misc_feature    2718..2774
                     /label=polylinker
                     /note="polylinker"
     primer_bind     complement(2787..2803)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2811..2827
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2835..2865)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2880..2901
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    complement(2894..3309)
                     /label=E. coli 5s rRNA and terminators
                     /note="E. coli 5s rRNA and terminators"
     regulatory      complement(2984..3011)
                     /regulatory_class="terminator"
                     /note="T2"
     regulatory      complement(3018..3143)
                     /regulatory_class="terminator"
                     /note="T1"
     rRNA            complement(3022..3309)
                     /product="5S ribosomal RNA"
     rep_origin      complement(3189..3777)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3948..4808)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AAA57326.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3948..4808)
                     /gene="bla"
                     /label=bla
     promoter        complement(4809..4913)
                     /gene="bla"
                     /label=AmpR promoter