LOCUS       Exported                5823 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pWW3804, complete sequence.
ACCESSION   KY776520
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Whitaker WR, Shepherd ES, Sonnenburg JL.
  TITLE     Tunable expression tools enable single-cell strain distinction in 
            the gut microbiome
  JOURNAL   Cell (2017) In press
REFERENCE   2  (bases 1 to 5823)
  AUTHORS   Whitaker WR, Shepherd ES, Sonnenburg JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAR-2017) Microbiology & Immunology, Stanford 
            University School of Medicine, 299 Campus Drive, Stanford, CA 
            94305-5124, USA
REFERENCE   3  (bases 1 to 5823)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5823
                     /organism="Cloning vector pWW3804"
                     /mol_type="other DNA"
                     /db_xref="taxon:1977346"
     misc_feature    17..284
                     /label=split antibiotic resistance marker
                     /note="split antibiotic resistance marker"
     promoter        complement(285..389)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(808..2733)
                     /codon_start=1
                     /product="TetQ"
                     /label=TetQ
                     /protein_id="ARI71372.1"
                     /translation="MNIINLGILAHIDAGKTSVTENLLFASGATEKCGCVDNGDTITDS
                     MDIEKRRGITVRASTTSIIWNGVKCNIIDTPGHMDFIAEVERTFKMLDGAVLILSAKEG
                     IQAQTKLLFNTLQKLQIPTIIFINKIDRAGVNLERLYLDIKANLSQDVLFMQNVVDGSV
                     YPVCSQTYIKEEYKEFVCNHDDNILERYLADSEISPADYWNTIIALVAKAKVYPVLHGS
                     AMFNIGINELLDAITSFILPPASVSNRLSSYLYKIEHDPKGHKRSFLKIIDGSLRLRDV
                     VRINDSEKFIKIKNLKTINQGREINVDEVGANDIAIVEDMDDFRIGNYLGAEPCLIQGL
                     SHQHPALKSSVRPDRPEERSKVISALNTLWIEDPSLSFSINSYSDELEISLYGLTQKEI
                     IQTLLEERFSVKVHFDEIKTIYKERPVKKVNKIIQIEVPPNPYWATIGLTLEPLPLGTG
                     LQIESDISYGYLNHSFQNAVFEGIRMSCQSGLHGWEVTDLKVTFTQAEYYSPVSTPADF
                     RQLTPYVFRLALQQSGVDILEPMLYFELQIPQAASSKAITDLQKMMSEIEDISCNNEWC
                     HIKGKVPLNTSKDYASEVSSYTKGLGIFMVKPCGYQITKGGYSDNIRMNEKDKLLFMFQ
                     KSMSSK"
     rep_origin      3323..4096
     rep_origin      3327..3715
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            3886..3995
                     /note="incP origin of transfer"
     terminator      4151..4177
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     regulatory      4152..4181
                     /regulatory_class="terminator"
     rep_origin      4184..4947
     rep_origin      complement(4283..4871)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4948..5821
                     /label=Chloramphenicol resistance cassette
                     /note="Chloramphenicol resistance cassette"
     CDS             5053..5712
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      5783..5817
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"