LOCUS       Exported                7212 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pWW102B, complete sequence.
ACCESSION   AY763107
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Wang W, Attia AS, Liu L, Rosche T, Wagner NJ, Hansen EJ.
  TITLE     Development of a shuttle vector for Moraxella catarrhalis
  JOURNAL   Plasmid 55 (1), 50-57 (2006)
  PUBMED    16188314
REFERENCE   2  (bases 1 to 7212)
  AUTHORS   Wang W, Attia AS, Liu L, Rosche T, Wagner NJ, Hansen EJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2004) Microbiology, University of Texas 
            Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 
            75390, USA
REFERENCE   3  (bases 1 to 7212)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="Shuttle vector pWW102B"
                     /mol_type="other DNA"
                     /db_xref="taxon:312804"
     rep_origin      302..1214
     rep_origin      complement(588..1133)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             1661..2335
                     /codon_start=1
                     /product="spectinomycin adenyltransferase"
                     /label=spectinomycin adenyltransferase
                     /note="spectinomycin resistance"
                     /protein_id="AAX15693.1"
                     /translation="MFGSGVESGLKPNSDLDFLVVVSEPLTDQSKEILIQKIRPISKKI
                     GDKSNLRYIELTIIIQQEMVPWNHPPKQEFIYGEWLQELYEQGYIPQKELNSDLTIMLY
                     QAKRKNKRIYGNYDLEELLPDIPFSDVRRAIMDSSEELIDNYQDDETNSILTLCRMILT
                     MDTGKIIPKDIAGNAVAESSPLEHRERILLAVRSYLGENIEWTNENVNLTINYLNNRLK
                     KL"
     CDS             complement(3743..5053)
                     /codon_start=1
                     /product="replication protein"
                     /label=replication protein
                     /note="pLQ510 replication protein"
                     /protein_id="AAX15694.1"
                     /translation="MSDFVQNASFYVEGKEIEIFKSNALVEGAFDLSPAEHDLMTLAIN
                     KLFSQGVGGKQVFISAKEFAIANQINENYAYEVLAETAKRLQKRTFKAKIYKDGLKKLV
                     GEEDPYSVARPKGDYELLDLHANWVQAVAYQEKSGFIYLMFSDVLAYLIQKTGEAYTKY
                     PYEKTIDLKRFHTKRLYELCCKRAGSKPPHGKKYPKVTMVVDEWKDFFGCINKYGAVND
                     FKRYVLTPVIKEINEQGEFELTFEQEKIGKIITHFTLIINDKRTKTAKIDKMINDSRDP
                     NTVDIIHGLTDKELAIVHQKVADYIVHLESKGELVNDFHRKNIEQKAIADRWGLDEHYE
                     QLQKAENERLARKEQAEREKQAKLAEQAEKQRQEAENAEFIAYFERLPQDEQTRIIDEV
                     GRTIEPFLKQFFDKSVKNGSSHKDLMYRGAFKQVMGV"
     CDS             complement(6512..7171)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /note="chloramphenicol resistance"
                     /protein_id="AAX15695.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(7172..62)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"